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    Home > Biochemistry News > Plant Extracts News > Real-Time PCR for the Quantification of Fungi In Planta

    Real-Time PCR for the Quantification of Fungi In Planta

    • Last Update: 2021-03-14
    • Source: Internet
    • Author: User
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    Methods enabling quantification of fungi in planta can be useful for a variety of applications. In combination with information on plant disease severity, indirect quantification of fungi in planta offers an additional tool in the screening of plants that are resistant to fungal diseases. In this chapter, a method is described for the quantification of
    from a fungus in plant leaves using real-time
    (qPCR). Although the method described entails quantification of the fungus
    Verticillium dahliae
    in lettuce leaves, the methodology described would be useful for other pathosystems as well. The method utilizes primers that are specific for amplification of a β-tubulin sequence from
    V. dahliae
    and a lettuce actin gene sequence as a reference for normalization. This approach enabled quantification of
    V. dahliae
    in the amount of 2.5 fg/ng of lettuce leaf DNA at 21 days following plant inoculation.
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