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lab conventional solution is always a lot less Some buffers,culture base, dyeing solution, antibiotic preparation, etc., here summarizes some conventional solution, cell chemistry and cell composition separation solution,and cell fusion solution, preparation of electron mirror specimen solution, development and shadow solution preparation. I hope it will be useful to everyone.
1, regular solution
(i)1/15mol/L PBS (Phosphate buffer, PBS) >
A liquid: 1/15mol/L Na2HPO4 solution
Na2HPO4 9.465g
distilled water to 1000ml
.B solution: l/15mol/L KH2PO4 solution
KH2P04 9.07g
Distilled water added to 1000m1
is packed in a brown bottle, stored in a 4c refrigerator, mixed in different proportions with time-A and B fluids, you can get the required pH buffer, see Table below:
. < "0" cellpadding,"0" cellspacing, "0" style, "color: rgb (0,0,0); line-height: 18.0p x; font-size: 12.0px; border-collapse: collapse; ">pH
liquid ml
B-liquid mI
pH
meth ml
. B-liquid mI
5.29
5.59
. 5.91
6.24
div style
< "text-left;" >6.64 2.5
5.0
. 10.0
20.0
30.0
40.0
97.5
90.0
80.0
70.0
60.0>60.0
< 6.81
6.98
. 7.17
7.38
7.73
8.04>
50.0
60.0
. 70.0
80.0
90.0
95.0
50.0
40.0
. 30.0
20.0
10.0
5.0
< "text-align: left;" > called Trypan blue powder 0.3 grams, dissolved in 100 ml of physiological salt water, heated to make it completely dissolved, with filter paper"> filterslag, Store at room temperature in a bottle.
(iii)0.5% phenol red indicator
phenol red 0.5g
0.1N (0.4%) NaOH 15ml
double steamed water 85 ml
places 0.5 grams of phenol red in the research, slowly adds 0.1NNaOH solution side grinding, and continuously sucks out the dissolved phenolic erythropoies until all dissolved, then adds 85 ml of bisfurent water, color is deep red, filtered by coarse filter paper to use, room temperature.
(4)5.6% NaHCO3 solution
said NaHCO35.6 g, dissolved in 100ml distilled water, stored at room temperature (if required can also be 10 pounds 15 minutes autocultension, 4 degrees C refrigerator storage)
(5)10 sg/ml autumn daffodils
autumn daffodils lOmg
physiological saline 100ml
is packed in a tea-colored bottle for storage fluid and stored in a refrigerator at 4C. Take 1 ml of storage fluid and 9 ml of physiological saline when you dump it.
(6)0.4% KCl-0.4% Sodium citrate low seepage
mixes 0.4% KCl and 0.4% sodium citrate in equal amounts, room temperature can be stored.
(seven) 2% sodium citrate
said sodium citrate 2 grams, plus 100 ml of double steamed water, room temperature preservation.
(eight) 0.2% methylan dye
< p style s"text-align:left;"> said the times.