Recovery rate and precision of 8 kinds of macrolides and lincosamide drug residues in puffer fish and eels

6.
2.
4.
9 Method recovery and precision

Use puffer fish samples without 8 kinds of antibiotics for addition recovery and precision experiments.
After adding 2.
0μg/kg, 4.
0μg/kg, 8.
0μg/kg, 10.
0μg/kg different concentration standards to the samples, shake them up to make the standards The sample is fully absorbed, and then extracted, purified, and determined according to this method.
The recovery rate and precision are shown in Table 6-34
.

Table 6-34 The recovery rate and precision experimental data of 8 kinds of antibiotics (n=10)

6.
2.
4.
10 Applicability of the method

Using this method, add recovery rate experiments for 8 antibiotics at four levels of 1LOD, 2LOD, 4LOD, and 10LOD for puffer fish and eels.
The analysis results are shown in Table 6-35 and Table 6-36
.

Table 6-35 Applicability of the method Addition recovery rate (%, n=10)

Table 6-36 Experimental data of recovery rate and precision of 8 kinds of antibiotics (eel, %)

6.
2.
4.
11 Repeatability and reproducibility

Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
.

Table 6-37 Measured substance content range, repeatability limit and reproducibility limit equation