Recovery rate and precision of the determination method of 5 cephalosporin residues in puffer fish and eels

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9 Method recovery and precision

Use samples that do not contain 5 cephalosporins to perform addition recovery and precision experiments.
After adding 4 different levels of 5 cephalosporin standards at 2-100μg/kg (LOD～10 LOD) to the sample , shake well to make The standard substance and the sample are fully absorbed, and then extracted and purified according to this method, and measured with a high performance liquid chromatography-tandem mass spectrometer.
The recovery rate and precision are shown in Table 5-46
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Table 5-46 Experimental data of recovery rate of 5 cephalosporins in puffer fish and eels (n=10)

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10 Repeatability and reproducibility

Under repeatability test conditions, the absolute difference between the two independent test results obtained does not exceed the repeatability limit (r).
If the difference exceeds the repeatability limit (r), the test results should be discarded and the two individual tests should be completed again.
Determination
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Table 5-47 The content range of 5 cephalosporins and the reproducibility limit and reproducibility limit equation

Related links: Determination of 5 cephalosporins residues in puffer fish and eels and selection of analysis conditions