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In the latest research paper officially published in the top academic journal "Cell", a Chinese scientific research team demonstrated the three-dimensional cryo-EM (cryo-EM) structure of the key state of the new coronavirus "transcriptional replication complex" (RTC), revealing viral mRNA How to carry out the molecular mechanism of capping and proofreading
"MRNA capping and replication correction play a vital role in the replication and transcription of the new coronavirus
Simply put, capping refers to the addition of a cap structure to the front end of the viral mRNA, which can increase the stability of the mRNA and ensure subsequent protein translation; replication correction uses the protein in the transcription replication complex to quickly process the replication process Bases that are mismatched can be corrected at any time
Among the currently known RNA viruses, the new coronavirus is the largest genome.
In this study, in order to solve this mystery, scientists are based on previous work mechanisms such as capped intermediate state RTC (Cap(-1)'-RTC), polymerase-centered extension complex (EC), etc.
▲The three-dimensional mechanism based on Cap(0)-RTC clarified the molecular mechanism of capping and replication correction of the new coronavirus (picture source: reference [1])
The study revealed that nsp14 uses the C-terminal N7 methylase domain to complete the third key reaction of the mRNA capping process
Another important finding is that the Cap(0)-RTC form of transcriptional replication complex forms a stable dimer in solution
Note: The original text has been deleted
Reference
[1] Liming Yan et al.
[2] Academician Rao Zihe and researcher Lou Zhiyong team published collaborative research results to clarify the molecular mechanism of new coronavirus mRNA synthesis, genome replication correction and escape nucleoside antiviral drugs Retrieved June 29 2021 from https:// edu.
