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    Home > Active Ingredient News > Study of Nervous System > Research on the sustained-release NT-3 three-dimensional microenvironment supporting the self-organized development of neural stem cells into neural network tissues with repair purposes was published in Bioactive Materials

    Research on the sustained-release NT-3 three-dimensional microenvironment supporting the self-organized development of neural stem cells into neural network tissues with repair purposes was published in Bioactive Materials

    • Last Update: 2021-06-08
    • Source: Internet
    • Author: User
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    Traumatic spinal cord injury can lead to spinal cord tissue defects, and the harsh microenvironment of the injury poses a huge challenge to the regeneration of spinal cord tissue and the survival of transplanted cells.
    Since the transplantation of embryonic spinal cord tissue at the spinal cord injury site for nearly 30 years, the embryonic spinal cord neurons have been used to replace dead spinal cord neurons, forming a neuronal relay at the injury/transplantation site to repair the spinal cord The idea of ​​damage is gradually formed and verified.
    However, embryonic spinal cord tissue transplantation is subject to ethical restrictions and the difficulty of obtaining transplants, which does not meet the requirements of clinical transformation.
    How to effectively apply cytokines, biological materials and
    Stem cells and other new tissue engineering technologies are the key to researchers to overcome difficulties in constructing neural network tissues similar to embryonic spinal cords.

    Recently, the team of Professor Zeng Yuanshan of Zhongshan Medical College of Sun Yat-sen University revealed that the key molecule neurotrophin-3 (NT-3) was screened based on the bioinformatics of embryonic spinal cord development , and the sustained-release technology was used to induce the expression of its tyrosine kinase receptor.
    TrkC neural
    stem cells self-organize into neural network tissue in the three-dimensional scaffold material.
    This
    tissue engineering product can be functionally integrated with the host neural circuit after transplantation into a rat with a complete spinal cord transection.
    This treatment strategy is optimized for the construction of the microenvironment
    Stem cell- derived tissue-engineered neural networks provide new ideas for repairing central nervous system damage.
    On April 8, 2021, Professor Zeng Yuanshan’s team published an online publication entitled "An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem" in the international TOP journal Bioactive Materials (District 1, Chinese Academy of Sciences, IF=8.
    724).
    Cell-derived neural network tissue with efficacy in repairing spinal cord injury" research results.

    During the development of the embryonic spinal cord, the mechanism of neurogenesis involves the interaction between specific ligands and receptors, which helps guide the construction of tissue-engineered neural networks for transplantation and repair of the structure and function of the full-transected spinal cord .
    This study analyzed the single-cell map of the embryonic spinal cord and found that NT-3 and its receptor TrkC play an important role in the development and maturation of spinal cord neurons.
    Subsequently, a method of ligand-receptor reactivity was designed to promote the formation of neural network self-organization.
    By planting NSCs with high TrkC expression in the NT-3 release three-dimensional scaffold, they can differentiate into neurons with mature phenotype and electrophysiological function in the NT-3 enriched microenvironment.
    Importantly, as the culture time increases, exogenous NT-3, autocrine growth factors from stem cells and extracellular matrix (such as laminin, etc.
    ) together create a unique ecological niche.
    Such trophic factors, stem cell autocrine growth factors, and the extracellular matrix deposited on the scaffold are dynamically integrated to promote tissue-like assembly, and ultimately form neurons with the function of transmitting excitatory information and oligodendrocytes with the potential for myelination A neural network composed of glial cells, astrocytes, a small amount of
    stem cells and extracellular matrix.
    The neural network tissue is transplanted to the 2 mm defect area of ​​the rat spinal cord.
    It can be observed that the neural network tissue containing the niche can survive for a long time in the injury/transplantation area, maintain the differentiated phenotype of the relevant cells, and form a process.
    The myelin structure is touched.
    In addition, the host nerve fibers regenerate into the spinal cord injury/transplant area and the transplanted NSC-derived neurons form a synapse-like structure, which promotes the improvement of the motor function of the paralyzed hind limbs of the rat.

    This research focuses on the need to improve the microenvironment of spinal cord injury areas and rebuild the spinal cord neural circuit.
    It is the first to simulate the mechanism of inducing neurons between ligands and receptors during embryonic development.
    The four elements of tissue culture time are designed for tissue engineering, and precise engineering induction is achieved by optimizing biological materials and modifying stem cells to meet the needs of efficient tissue engineering to construct biological tissues.
    The application of
    tissue engineering neural network tissue transplantation can also avoid the risk of differentiation uncertainty, abnormal proliferation and heterotopic migration of direct transplantation of stem cells in the spinal cord injury area, and provides a new treatment plan for the repair of spinal cord injury.
    (
    Bioon.
    com)
    Traumatic spinal cord injury can lead to spinal cord tissue defects, and the harsh microenvironment of the injury poses a huge challenge to the regeneration of spinal cord tissue and the survival of transplanted cells.
    Since the transplantation of embryonic spinal cord tissue at the spinal cord injury site for nearly 30 years, the embryonic spinal cord neurons have been used to replace dead spinal cord neurons, forming a neuronal relay at the injury/transplantation site to repair the spinal cord The idea of ​​damage is gradually formed and verified.
    However, embryonic spinal cord tissue transplantation is subject to ethical restrictions and the difficulty of obtaining transplants, which does not meet the requirements of clinical transformation.
    How to effectively apply cytokines, biological materials and
    Stem cells and other new tissue engineering technologies are the key to researchers to overcome difficulties in constructing neural network tissues similar to embryonic spinal cords.
    Stem Cell Tissue Engineering


    Recently, the team of Professor Zeng Yuanshan of Zhongshan Medical College of Sun Yat-sen University revealed that the key molecule neurotrophin-3 (NT-3) was screened based on the bioinformatics of embryonic spinal cord development , and the sustained-release technology was used to induce the expression of its tyrosine kinase receptor.
    TrkC neural
    stem cells self-organize into neural network tissue in the three-dimensional scaffold material.
    This
    tissue engineering product can be functionally integrated with the host neural circuit after transplantation into a rat with a complete spinal cord transection.
    This treatment strategy is optimized for the construction of the microenvironment
    Stem cell- derived tissue-engineered neural networks provide new ideas for repairing central nervous system damage.
    On April 8, 2021, Professor Zeng Yuanshan’s team published an online publication entitled "An NT-3-releasing bioscaffold supports the formation of TrkC-modified neural stem" in the international TOP journal Bioactive Materials (District 1, Chinese Academy of Sciences, IF=8.
    724).
    Cell-derived neural network tissue with efficacy in repairing spinal cord injury" research results.
    Bioinformatics Stem Cell Tissue EngineeringStem Cell Tissue Engineering


    During the development of the embryonic spinal cord, the mechanism of neurogenesis involves the interaction between specific ligands and receptors, which helps guide the construction of tissue-engineered neural networks for transplantation and repair of the structure and function of the full-transected spinal cord .
    This study analyzed the single-cell map of the embryonic spinal cord and found that NT-3 and its receptor TrkC play an important role in the development and maturation of spinal cord neurons.
    Subsequently, a method of ligand-receptor reactivity was designed to promote the formation of neural network self-organization.
    By planting NSCs with high TrkC expression in the NT-3 release three-dimensional scaffold, they can differentiate into neurons with mature phenotype and electrophysiological function in the NT-3 enriched microenvironment.
    Importantly, as the culture time increases, exogenous NT-3, autocrine growth factors from stem cells and extracellular matrix (such as laminin, etc.
    ) together create a unique ecological niche.
    Such trophic factors, stem cell autocrine growth factors, and the extracellular matrix deposited on the scaffold are dynamically integrated to promote tissue-like assembly, and ultimately form neurons with the function of transmitting excitatory information and oligodendrocytes with the potential for myelination A neural network composed of glial cells, astrocytes, a small amount of
    stem cells and extracellular matrix.
    The neural network tissue is transplanted to the 2 mm defect area of ​​the rat spinal cord.
    It can be observed that the neural network tissue containing the niche can survive for a long time in the injury/transplantation area, maintain the differentiated phenotype of the relevant cells, and form a process.
    The myelin structure is touched.
    In addition, the host nerve fibers regenerate into the spinal cord injury/transplant area and the transplanted NSC-derived neurons form a synapse-like structure, which promotes the improvement of the motor function of the paralyzed hind limbs of the rat.
    Tissue Engineering Stem Cell


    This research focuses on the need to improve the microenvironment of spinal cord injury areas and rebuild the spinal cord neural circuit.
    It is the first to simulate the mechanism of inducing neurons between ligands and receptors during embryonic development.
    The four elements of tissue culture time are designed for tissue engineering, and precise engineering induction is achieved by optimizing biological materials and modifying stem cells to meet the needs of efficient tissue engineering to construct biological tissues.
    The application of
    tissue engineering neural network tissue transplantation can also avoid the risk of differentiation uncertainty, abnormal proliferation and heterotopic migration of direct transplantation of stem cells in the spinal cord injury area, and provides a new treatment plan for the repair of spinal cord injury.
    (
    Bioon.
    com)
    Tissue Engineering Stem Cell
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