Research progress on rapid detection methods of bovine milk somatic cells (1)

With the improvement of people's living standards, residents' demand for dairy products has increased year by year

1.

Somatic cell count (SCC) in milk refers to the total number of cells per milliliter of milk

2.

(1) Microscope counting method

The method of counting somatic cells in milk with a microscope is to spread 10uL of milk evenly on an area of ​​1cm² with a micro-syringe and a standard template, and then place it under a microscope after dyeing and drying steps

(2) Coulter counter measurement method

The principle of the Coulter counter is to use voltage changes to form pulses, and count by counting the number of pulses

The Coulter counter is equipped with two electrodes.

(3) High-speed body paper cell counter

High-speed somatic cell counters currently mainly include Bently counters from the United States, Fossomatic5000 series milk somatic cell analyzers from FOSS of Denmark, and Dclta somatic cell juice counters from the Netherlands

The principle of high-speed somatic cell counter measurement is to stain the measured somatic cells with fluorescent dyes in advance and place them in a suspension liquid, and then chase the somatic cells one by one through a very narrow gap channel

(4) CMT test

The principle of the CMT method is to use surfactants to destroy somatic cells, so that DNA and other nucleic acid substances are released to produce an agglutinated state.