Researchers from the Feed Research Institute of the Chinese Academy of Agricultural Sciences have innovated the method for the isolation and culture of primary cells from duck embryo liver

Recently, researchers from the Institute of Feed Research, Chinese Academy of Agricultural Sciences have developed a simple and rapid method for the isolation and culture of primary duck embryo liver cells, and studied gene alternative splicing in the choline deficiency model of duck embryo liver primary cells.
, put forward new insights for the study of the correlation between duck choline and gene structure, and the relevant research results were published in "Animal Bioscience"
.

 

This study innovatively optimized the method for isolating primary liver cells from the aspects of duck embryo age, separation method, digestion method, and digestive juice concentration, and evaluated the separation efficiency from the aspects of cell viability, cell purity and cell function.
RT-PCR was used for detection.
The expression of AFP and ALB genes in cells was used to identify primary duck embryo liver cells, and finally a simple, economical and rapid method for isolation and culture of duck embryo liver primary cells was formed, and high purity and high activity duck embryo liver primary cells were obtained.
cells
.
Based on this, the effect of choline on lipid metabolism in primary cells of duck embryo liver was further verified and studied, and a choline deficiency model in primary cells of duck embryo liver was established, and choline was studied by RNA-seq and rMATS technology.
The lack of abnormal variable splicing in the model enriches the theory of choline nutrition and provides new ideas for the molecular mechanism of choline regulating fat metabolism in ducks
.

 

This research was funded by the National Natural Science Foundation of China (31802081)
.

 

Article link: "10.
5713/ab.
22.
0051