Revealed histone partners regulate the mechanism of neural stem cells.

The cerebral cortex is a highly developed central region in the mammalian brain, responsible for controlling important functions such as cognition, memory and emotional behavior.
normal embryonic cerebral cortex development is very important to maintain the function of the cortex, and it is of great significance to gain a comprehensive understanding of the mechanism and regulatory mechanism of the cerebral cortex of the embryo.
the process of embryonic cerebral cortex development is precisely regulated by a variety of signaling molecules inside and outside the cell to ensure the normal development of the brain timing.
epigenetic control is the key regulatory factor in the development of the cortex.
histone companion NapNap1l1 and histone binding to participate in the assembly and deassembly of chromatin, the stability of chromatin structure and gene expression regulation has important functions, in the cell cycle regulation, cell polarity and histone transport page plays an essential role, but in the brain development process of neural stem cell proliferation and differentiation process of the function and mechanism is unknown.
recently, the Institute of Zoology of the Chinese Academy of Sciences, jia Jianwei research team in determining the Nap1l1 embryo brain development process expression changes and distribution, through the embryo electro-transfer experiment found that Nap1l1 after the fall of neural stem cell proliferation decreased, neuron differentiation level increased.
Nap1l1 knockout mice, built by CRISPR-Cas9 technology, further determined their pronosibles.
results show that Nap1l1 can be combined with SETD1A to promote the level of H3K4me3 on the promoter of the downstream gene RassF10, thereby promoting the expression of RassF10.
expression of RassF10 can save the abnormality of cell distribution, proliferation and differentiation during embryo development caused by Nap1l1 knockdown.
the study revealed the important role of histone molecular companion Nap1l1 in the regulation of neural stem cells in early embryonic brain development, and helped to explore the pathology of Nap1l1-related diseases.
related research published in Cell Reports, animal stem cells and reproductive biology national key laboratory master's student Qiao Huimin, doctoral student Li Wei as co-first author, researcher Jia Jianwei, assistant researcher Ji Fen as co-communication author.
the research has been supported by key projects of the National Natural Science Foundation of China, key basic research projects of the Ministry of Science and Technology, and special projects of strategic leading science and technology of the Chinese Academy of Sciences.
.