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    Home > Biochemistry News > Biotechnology News > Reverse cross-level tool virus H129-G4 and single-stage tool virus H129-TK-tdT.

    Reverse cross-level tool virus H129-G4 and single-stage tool virus H129-TK-tdT.

    • Last Update: 2020-09-01
    • Source: Internet
    • Author: User
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    It is one of the important tasks of neuroscience research to analyze the neural loop connection between different brain regions and different types of neurons.
    But at present, the development of the inward tracer tool virus used to study the output neural loop is slow, and the reverse cross-level tool used to finely study the direct output network has not been studied.
    Recently, Luo Minhua, a researcher at the Wuhan Institute of Virus Research of the Chinese Academy of Sciences, collaborated with Xu Fuqiang, a researcher at the Wuhan Institute of Physics and Mathematics, to report on the more effective cross-level tool virus H129-G4 and the first straight-forward tool virus H129-TK-tdT that can be used to trace direct output loops. The results of the
    study were published online recently in Molecular Neurodegeneration, with assistant researcher Zeng Wenbo and Ph.D. student Jiang Haifei as co-authors of the paper and Wuhan Viruses' Solomon Hua, Zhao Fei and Wuhan Institute Xu Fuqiang as co-authors.
    using herpes simplex virus type 1, HSV-1 H129 strains of human co-chromosomes as a genetic modification platform, the researchers inserted a multi-copy GFP-coded protein into the H129 genome to build H129-G4, knocking out the TK gene to build H129-TK-tdT.
    microfluidic chip (Microfluidic system) and in vivo visual neural loop tests showed that the resulting viral tool was transmitted only across synapses. The
    H129-G4 injection into the primary motor cortical layer (M1) in mice successfully traced the multistage output loop of M1 and clearly demonstrated the details of individual neuron morphology, with marking brightness that meets the requirements of micro-optical sliced fault imaging (fMOST) and can be used for brain-scale reconstruction of neural loop connections.
    The first reverse-cross single synaptic marker system consisting of H129-TK-tdT and auxiliary viruses has also succeeded in marking visual information conduction loops, the theotynapsy to the ontological sensory cortical conduction loops, and the middle brain dopamine neuron projection loops.
    these tools largely complement the existing neural loop tracing tool set.
    research has been supported by the Center for Excellence in Innovation in Brain Science and Intelligent Technology of the Chinese Academy of Sciences, the National Natural Science Foundation of China and the National Key Basic Research and Development Program ("973").
    Wuhan Virus is the first complete unit, the main participating units include: The Chinese Academy of Sciences Wuhan Institute, Wuhan Photoelectric National Laboratory, Peking University School of Life Sciences, Beijing Normal University Institute of Brain and Cognitive Sciences and Kunming University of Technology.
    , the virus tracing tool developed by the study has been used by several neuroscience collaborating laboratories and has been published in collaboration articles, including Nature Neuroscience.
    .
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