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Reverse Transcription-PCR for Phytoplasma Detection Utilizing Crude Sap Extractions

 Last Update: 2021-03-11
 Source: Internet
 Author: User

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methylation specific pcr

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Phytoplasmas are routinely detected by nucleic acid-based techniques. These approaches rely on enriched phytoplasma
DNA
extracts of good quality, following labor intensive and time-consuming purification protocols. Here we describe a very rapid, specific, sensitive, and reliable method for flavescence dor�e phytoplasma detection, based on real-time Taqman
�
reverse transcription-
PCR
of the 16S rRNA. The protocol is particularly useful for large-scale screening of vineyards and nurseries, pathogen surveys, and field epidemiological studies.

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