Rice transcription factor OsWRKY53 is able to regulate BR signals and rice strains in a forward way.

BR is an important sterol plant hormone that is involved in regulating all aspects of plant growth and development, including regulating plant strains, cell division, cell elongation, differentiation of vascular beams, the construction of light forms, and response to various biological and non-biological stress.
BR signal elements and signal transdulsion paths have been studied more clearly in the gemon leaf model plant auspicine mustard, while in the single leaf model plant rice research is relatively small, rice BR signal elements and regulatory networks need to be excavated and clarified.
For the first time, researchers at the Northeast Institute of Geography and Agricultural Ecology of the Chinese Academy of Sciences have found that the rice transcription factor OsWRKY53 is able to regulate BR signals positively, and the OsWRKY53 gene over-expression of genetically modified rice (OsWRKY53-OE) shows increased leaf inclination, increased seed growth, and external leaf inclination. The BR processing supersensitivity of the source and a series of BR signal-enhanced physiomes, while knockout mutants (oswrky53) show a series of BR signal defects such as smaller leaf inclination, smaller seeds, taller and shorter plants, and decreased BR processing sensitivity of their leaf inclination to external sources.
that OsWRKY53 is a new positive regulator of BR signals.
further research and analysis found that there is a relationship between OsWRKY53 and the fissilegen-activated protein kinase OsMAPK6, and OsMAPK6 is able to phosphorulate OsWRKY53.
analysis of inosometer kinase determination found that Ser in the SP cluster sequence at the OsWRKY53N end is the main OsMAPK6 phosphorylation point.
after the Ser was mutated into Ala, OsWRKY53(SA) over-expression of genetically modified rice showed a similar ideotype to wild type because it could not be phosphorylated by OsMAPK6, and after Ser was mutated into Asp, OsWRKY53 (SD) over-expression of genetically modified rice showed a more significant BR signal enhancement.
that OSWRKY53 is phosphorylated by OSMAPK6 is the key to its function as a positive regulatory factor for BR signals. the
study reveals that the rice transcription factor OsWRKY53 can regulate BR signal and rice strains forward, which not only enriches the BR signal transdulation pathway, but also excavates the unreported biological function of OsWRKY53, and provides new research ideas and research perspectives for signal recognition, signal communication and signal transdulation between different hormones.
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