Ring dimension boxwood D

Ring dimension boxwood D

Cyclovirobuxine

【Structural formula】

[Molecular formula and molecular weight] C ₂₆ H ₄₆ N ₂ O; 402.

[Source] Buzus microph ylla Sieb.

【Properties】Colorless needle-like crystals

This product is soluble in chloroform , methanol , ethanol

Melting point: 217.

【Purity Check】

TLC

1.

Developing solvent ethyl acetate - methanol - conc.

Check the color development of dilute bismuth potassium iodide, inspect under sunlight

2.

Developing solvent of chloroform - acetone - diethylamine (5: 4: 0.

Check the color development of dilute bismuth potassium iodide, inspect under sunlight

High performance liquid chromatography

Chromatographic column AgilentSB C ₁₈ , 10um (4.

Mobile phase acetonitrile-10mmol potassium dihydrogen phosphate (5% phosphoric acid adjusted to pH 7.

Detection wavelength 203nm

[Structural identification] 202nm

3307(-OH), 2939(-CH), 1491, 1450, 1346, 1153, 1093, 1034, 773, 710

EI-MS m/z: 402[M]+

¹ H-NMR (CD ₃ OD) δ: 0.

¹³ C-NMR (CD ₃ OD) δ: 31.
7(C-1), 32.
6(C-2), 68.
6(C-3), 39.
9(C-4), 47.
3(C-5), 21.
3(C- 6), 26.
9(C-7), 48.
5(C-8), 19.
4(C-9), 26.
2(C-10), 25.
8(C-11), 32.
6(C-12), 44.
7(C-13) ), 47.
3(C-14), 45.
1(C-15), 78.
7(C-16), 62.
5(C-17), 18.
6(C-18), 30.
3(C-19), 44.
7(C-20) , 15.
0(C-21), 25.
7(C-22), 19.
2(C-23), 58.
9(C-24), 18.
6(C-25), 39.
9(C-26)
.

【Storage】Dry and airtight
.

Related link: Uridine