S1 Mapping Using Single-Stranded DNA Probes
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Last Update: 2020-11-23
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Source: Internet
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Author: User
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The S1 nuclease is an endonuclease isolated from
Aspergillus oryzae
that digests single- but not double-stranded nucleic acid. In addition, it digests partially mismatched double-stranded molecules with such sensitivity that even a single base-pair mismatch can be cut and hence detected. In practice, a probe of end-labeled double-stranded
DNA
is denatured and hybridized to complementary RNA molecules. S1 is used to recognize and cut mismatches or unannealed regions and the products are analyzed on a denaturing polyacrylamide gel. A number of different uses of the S1 nuclease have been developed to analyze mRNA taking advantage of this property (
1
,
2
). Both qualitative and quantitative information can be obtained in the same experiment (
3
).
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