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    Home > Biochemistry News > Biotechnology News > S1 Mapping Using Single-Stranded DNA Probes

    S1 Mapping Using Single-Stranded DNA Probes

    • Last Update: 2020-11-23
    • Source: Internet
    • Author: User
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    The S1 nuclease is an endonuclease isolated from
    Aspergillus oryzae
    that digests single- but not double-stranded nucleic acid. In addition, it digests partially mismatched double-stranded molecules with such sensitivity that even a single base-pair mismatch can be cut and hence detected. In practice, a probe of end-labeled double-stranded
    DNA
    is denatured and hybridized to complementary RNA molecules. S1 is used to recognize and cut mismatches or unannealed regions and the products are analyzed on a denaturing polyacrylamide gel. A number of different uses of the S1 nuclease have been developed to analyze mRNA taking advantage of this property (
    1
    ,
    2
    ). Both qualitative and quantitative information can be obtained in the same experiment (
    3
    ).
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