-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
- Cosmetic Ingredient
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
A non-profit academic platform initiated by overseas returnees to share information, integrate resources and exchange academics, occasionally Fengyue's new crown research team from Rutgers University in the United States invented a new method that can safely and effectively boost the immune response generated by nucleic acid vaccines
.
The method they use is similar in form to traditional cupping therapy
.
In the experiment, the team first injected a new crown deoxyribonucleic acid (DNA) vaccine into the skin of mice, and then applied negative pressure to the epidermis to make the mice produce powerful, 100 times stronger than pure injection of nucleic acid vaccine Immune response
.
Based on this encouraging result, GeneOne Life Science, the sponsor of the research project, obtained technology authorization and launched human clinical trials of the new crown DNA vaccine
.
Up to now, clinical data has confirmed the safety of this technology on the human body and the effectiveness of the immune response
.
Especially in terms of T cell immune response, the effect of this method surpasses the existing methods and means
.
At present, the clinical trial has successfully entered the second phase
.
The results of the mouse experiment were published in Science Advances on November 5; subsequent immunological animal models and clinical results will be published in other journals
.
Figure 1.
A.
Experimental mouse model
.
After the DNA molecule is injected, a short negative pressure is applied at the injection point to help the molecule enter the skin cell to achieve transfection
.
B.
Negative pressure is achieved by connecting a small cup to the vacuum pump
.
C.
Because the pressure is moderate and short-lived, there is no bruise on the skin of the mouse after the pressure is applied
.
The main person in charge of the project, Professor Lin Hao from the Department of Mechanical Engineering of Rutgers University, said: “This method is completely non-invasive
.
For the field of nucleic acid therapy and nucleic acid vaccines, this method provides a simple and inexpensive method.
It is also an easy-to-scale delivery platform
.
"Nucleic acid therapy is a new generation of biomedical technology, which mainly uses biologically functional molecules such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) that encode genetic information for disease treatment and vaccines.
There are attractive prospects in other fields
.
In the current COVID-19 pandemic, this technology platform has shown great power: several nucleic acid-based vaccines (such as Pfizer, Modena, Johnson & Johnson, etc.
) have been rapidly developed, manufactured and distributed to the community, which has played a role in blocking the spread of COVID-19.
The key role
.
The main mechanism for nucleic acid therapy to take effect comes from the protein encoded by the nucleic acid, such as the spike protein on the new coronavirus
.
When the nucleic acid enters the host cell, it will use the inherent mechanisms and resources in the cell to "copy" a large amount of the encoded protein
.
Taking the new crown vaccine as an example, when the host cell produces the virus's spike protein under the instructions of the nucleic acid encoding, it will trigger the body's immune mechanism
.
The resulting immune response and neutralizing antibodies will play a protective role in the next virus invasion
.
The spike protein is just a small fragment of the virus, and it does not cause disease like a virus
.
Therefore, the key step for this type of therapy to take effect is also one of the key bottlenecks of nucleic acid therapy, which is the so-called in vivo transfection: nucleic acid molecules need to cross the cell membrane barrier in the human body to enter the internal "factory" of the cell.
The purpose of producing the functional protein it encodes
.
But if there is no other means for blessing, and only pure DNA and RNA molecules are injected into the tissue, they will not automatically enter the inside of the host cell, but will float outside the cell and quickly degrade and fail
.
The current mRNA vaccines use lipid nanoparticles to encapsulate these nucleic acid molecules, which can not only resist degradation, but also assist them to cross the host cell membrane and enter the cell
.
For DNA nucleic acid molecules, electrotransfection is currently the most commonly used, that is, by applying a strong but instantaneous electric field to open the channel to the inside of the cell
.
However, this type of method often has great side effects, such as inflammation, pain, and tissue damage
.
In the new method reported this time, after the injection of the pure DNA preparation, the researchers just apply a negative pressure at the injection site
.
It is the physical and biochemical processes initiated by this negative pressure that help nucleic acid molecules to cross the cell wall barrier
.
Professor Lin Hao commented: “The method is simple, painless and without any observable side effects so far
.
” This method is similar to cupping: the traditional cupping is to place a small pot that is heated and cooled on the skin to produce negative effects .
Pressure, so as to achieve the purpose of increasing blood circulation and promoting the body's self-repair
.
Figure 2.
Above: A portable, ordinary battery-driven negative pressure device used in human clinical trials; only one button is required to operate
.
Bottom: Device applied to the human epidermis (Image source: Maria Atzampou, Rutgers University)
.
The inset shows the 100-fold immune gain effect caused by this method
.
"Transfection technology is a very critical step for nucleic acid therapy to enter a wide range of clinical applications; vaccines are only one of the application prospects of nucleic acid therapy
.
" Professor Lin Hao said
.
"Our new technology is safe and efficient, and it has the advantages of being cheap, easy to apply on a large scale, and easy to operate
.
In the subdivision of nucleic acids, compared with mRNA, DNA-based technology also has its own advantages, such as not requiring very Strict cold chain transportation conditions
.
DNA preparations can be kept at room temperature for a long time, so it is easier to extend the antennae to remote and resource-poor areas
.
" He also said: "Although we currently mainly try DNA molecular transfection And vaccines, but it does not rule out that this method has similar high-efficiency transfection effects on RNA-based nucleic acids
.
"This work comes from the cooperation of Rutgers University and GeneOne Life Science
.
The Rutgers University team is led by Professor Lin Hao, including Professor Jonathan Singer and Professor Jerry Shan from the Department of Mechanical Engineering; Professor David Shreiber and Professor Jeffrey Zahn from the Department of Biomedical Engineering; and Emran Lallow, Nandita Jhumur, Sarah Park, Julie Melnik, etc.
PhD students and researchers
.
*The Science and Technology Communication Center of the China Association for Science and Technology supports the 2021 China Science and Technology Achievement Promotion Program in the Knowledge Society Academic Circle.
Thesis information: Lallow, EO, Jhumur, NC, Ahmed, I.
, Kudchodkar, SB, Roberts, CC, Melnik, JM, Park, S .
, Muthumani, K.
, Shan, JW, Zahn, JD, Shreiber, DI, Singer, JP, Park, YK, Maslow, JN and Lin, H.
2021 “Novel suction-based in vivo transcutaneous DNA transfection platform,” Science Advances 7, eabj0611.
Link to the paper: https:// Click on the Zhishe Talent Square below to view the latest academic recruitment
.
The method they use is similar in form to traditional cupping therapy
.
In the experiment, the team first injected a new crown deoxyribonucleic acid (DNA) vaccine into the skin of mice, and then applied negative pressure to the epidermis to make the mice produce powerful, 100 times stronger than pure injection of nucleic acid vaccine Immune response
.
Based on this encouraging result, GeneOne Life Science, the sponsor of the research project, obtained technology authorization and launched human clinical trials of the new crown DNA vaccine
.
Up to now, clinical data has confirmed the safety of this technology on the human body and the effectiveness of the immune response
.
Especially in terms of T cell immune response, the effect of this method surpasses the existing methods and means
.
At present, the clinical trial has successfully entered the second phase
.
The results of the mouse experiment were published in Science Advances on November 5; subsequent immunological animal models and clinical results will be published in other journals
.
Figure 1.
A.
Experimental mouse model
.
After the DNA molecule is injected, a short negative pressure is applied at the injection point to help the molecule enter the skin cell to achieve transfection
.
B.
Negative pressure is achieved by connecting a small cup to the vacuum pump
.
C.
Because the pressure is moderate and short-lived, there is no bruise on the skin of the mouse after the pressure is applied
.
The main person in charge of the project, Professor Lin Hao from the Department of Mechanical Engineering of Rutgers University, said: “This method is completely non-invasive
.
For the field of nucleic acid therapy and nucleic acid vaccines, this method provides a simple and inexpensive method.
It is also an easy-to-scale delivery platform
.
"Nucleic acid therapy is a new generation of biomedical technology, which mainly uses biologically functional molecules such as deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) that encode genetic information for disease treatment and vaccines.
There are attractive prospects in other fields
.
In the current COVID-19 pandemic, this technology platform has shown great power: several nucleic acid-based vaccines (such as Pfizer, Modena, Johnson & Johnson, etc.
) have been rapidly developed, manufactured and distributed to the community, which has played a role in blocking the spread of COVID-19.
The key role
.
The main mechanism for nucleic acid therapy to take effect comes from the protein encoded by the nucleic acid, such as the spike protein on the new coronavirus
.
When the nucleic acid enters the host cell, it will use the inherent mechanisms and resources in the cell to "copy" a large amount of the encoded protein
.
Taking the new crown vaccine as an example, when the host cell produces the virus's spike protein under the instructions of the nucleic acid encoding, it will trigger the body's immune mechanism
.
The resulting immune response and neutralizing antibodies will play a protective role in the next virus invasion
.
The spike protein is just a small fragment of the virus, and it does not cause disease like a virus
.
Therefore, the key step for this type of therapy to take effect is also one of the key bottlenecks of nucleic acid therapy, which is the so-called in vivo transfection: nucleic acid molecules need to cross the cell membrane barrier in the human body to enter the internal "factory" of the cell.
The purpose of producing the functional protein it encodes
.
But if there is no other means for blessing, and only pure DNA and RNA molecules are injected into the tissue, they will not automatically enter the inside of the host cell, but will float outside the cell and quickly degrade and fail
.
The current mRNA vaccines use lipid nanoparticles to encapsulate these nucleic acid molecules, which can not only resist degradation, but also assist them to cross the host cell membrane and enter the cell
.
For DNA nucleic acid molecules, electrotransfection is currently the most commonly used, that is, by applying a strong but instantaneous electric field to open the channel to the inside of the cell
.
However, this type of method often has great side effects, such as inflammation, pain, and tissue damage
.
In the new method reported this time, after the injection of the pure DNA preparation, the researchers just apply a negative pressure at the injection site
.
It is the physical and biochemical processes initiated by this negative pressure that help nucleic acid molecules to cross the cell wall barrier
.
Professor Lin Hao commented: “The method is simple, painless and without any observable side effects so far
.
” This method is similar to cupping: the traditional cupping is to place a small pot that is heated and cooled on the skin to produce negative effects .
Pressure, so as to achieve the purpose of increasing blood circulation and promoting the body's self-repair
.
Figure 2.
Above: A portable, ordinary battery-driven negative pressure device used in human clinical trials; only one button is required to operate
.
Bottom: Device applied to the human epidermis (Image source: Maria Atzampou, Rutgers University)
.
The inset shows the 100-fold immune gain effect caused by this method
.
"Transfection technology is a very critical step for nucleic acid therapy to enter a wide range of clinical applications; vaccines are only one of the application prospects of nucleic acid therapy
.
" Professor Lin Hao said
.
"Our new technology is safe and efficient, and it has the advantages of being cheap, easy to apply on a large scale, and easy to operate
.
In the subdivision of nucleic acids, compared with mRNA, DNA-based technology also has its own advantages, such as not requiring very Strict cold chain transportation conditions
.
DNA preparations can be kept at room temperature for a long time, so it is easier to extend the antennae to remote and resource-poor areas
.
" He also said: "Although we currently mainly try DNA molecular transfection And vaccines, but it does not rule out that this method has similar high-efficiency transfection effects on RNA-based nucleic acids
.
"This work comes from the cooperation of Rutgers University and GeneOne Life Science
.
The Rutgers University team is led by Professor Lin Hao, including Professor Jonathan Singer and Professor Jerry Shan from the Department of Mechanical Engineering; Professor David Shreiber and Professor Jeffrey Zahn from the Department of Biomedical Engineering; and Emran Lallow, Nandita Jhumur, Sarah Park, Julie Melnik, etc.
PhD students and researchers
.
*The Science and Technology Communication Center of the China Association for Science and Technology supports the 2021 China Science and Technology Achievement Promotion Program in the Knowledge Society Academic Circle.
Thesis information: Lallow, EO, Jhumur, NC, Ahmed, I.
, Kudchodkar, SB, Roberts, CC, Melnik, JM, Park, S .
, Muthumani, K.
, Shan, JW, Zahn, JD, Shreiber, DI, Singer, JP, Park, YK, Maslow, JN and Lin, H.
2021 “Novel suction-based in vivo transcutaneous DNA transfection platform,” Science Advances 7, eabj0611.
Link to the paper: https:// Click on the Zhishe Talent Square below to view the latest academic recruitment
