Sand and soil tube method of microbial species preservation method.

Channel News: Sand and soil pipe law is a kind of carrier preservation law.
the cultured microbial cells or spores with sterile water to make suspension, mixed evenly into the sterilized sand pipe, or directly scraped mature spores in the sterilized sand pipe, so that microbial cells or spores adsorption on the sand carrier, water in the tube after drying the fuse seal or dryer in 4 to 6 degrees C or room temperature preservation method.
operation steps are as follows: 1, sand control ready to use 60 mesh screening of river sand, discarding large particles and impurities, and then 80 mesh screening, remove fine sand.
use iron-absorbing stone to absorb iron, put in a container with 10% hydrochloric acid immersion, such as more organic matter in river sand can be soaked with 20% hydrochloric acid.
24 hours after pouring hydrochloric acid, wash with water several times to neutral, dry or dry the sand.
take another 40 to 60 cm of soil under the ground where the non-farming layer is barren and less viscous, shredded, 100 mesh sieveed, washed to neutral and dried.
will be processed sand, soil by mass ratio of 2:1 mix.
mixed sand into an ampoule tube or small test tube, the height of about 1cm, stuffed cotton plugs, 121 degrees C wet heat sterilization 30min.
randomly extracted after sterilization of a number of sand pipe, sterile conditions to take a little sand to the nutrient gravy medium, 30 degrees C culture 24 hours, check the growth of no microorganisms can be used.
2, bevel culture preparation reference to the regular transplant method.
3, preparation bacteria suspension to the cultured slope culture into 3 to 5 mL sterile water, wash down cells or spores to make bacterial suspension.
sterile straw to absorb the bacteria suspension, evenly dripped into the sand pipe, each tube 0.2 to 0.5mL.
and mold can be directly picked spores mixed into the sand pipe.
4, dry vacuum to draw moisture from the sand pipe.
5, preservation The sand pipe is sealed with flame and stored in a low temperature (4 to 6 degrees C) dry place to preserve, every six months to check the survival and purity of the species.
or wrap the sand pipe directly in d'or plastic paper and store it in a dryer.
for 2 to 10 years.
6, restore culture under sterile conditions to open the sand pipe, take part of the sand grain on the appropriate sloped culture base, grow bacteria backward and then transfer again.
soil particles in a suitable liquid medium, multiply culture and then transfer the slope.
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