Scientific Reports: The use of a new generation of base sequence analysis method "long read sequencing" for the new coronavirus will greatly reduce time and cost

Recently, the research team of the University of Southern California (USC) School of Medicine SungYong Park and others have developed a faster and more accurate new coronavirus pneumonia ( COVID-19 ) gene analysis (sequence) technology
.

This result was published in "Scientific Reports".

COVID-19 New Coronary Pneumonia

Amino acid mutations, clades and lineage NP/OP specimens of 25 whole-genome sequences from COVID-19 remnants

Amino acid mutations, clades and lineage NP/OP specimens of 25 whole-genome sequences from COVID-19 remnants

The study, using this technology, successfully analyzed the base sequences of the viral genes of 25 people infected with the new coronavirus in Los Angeles, California, the United States from April to June last year
.

Previously, "short-read sequencing" was used to subdivide the new coronavirus gene into about 100 and analyze it, and then grasp the true face of the virus and whether it has mutated or not

Before infection , “short-read sequencing” is used to subdivide the new coronavirus genes into about 100 and analyze them, and then grasp the true face of the virus and whether it has mutated or not .
On the contrary, the research team divided the gene into three parts for analysis, which greatly reduced the time and cost .
There are expectations that it will shorten the sequencing time, which previously took about 10 days, to within a few days .
Previously, "short-read sequencing" was used to subdivide the new coronavirus gene into about 100 and analyze it, and then grasp the true face of the virus and whether it has mutated or not

For samples, the time required for CorvGenSurv is approximately 15 hours for library preparation and 30 hours for sequencing

The team developed this high-precision, cost-effective SARS-CoV-2 whole-genome sequencing platform for COVID-19 genome monitoring, called CorvGenSurv (coronavirus genome monitoring)
.

CorvGenSurv directly amplifies nasopharyngeal/oropharyngeal (NP/OP) swab specimens from patients with viral RNA from COVID-19 and sequenced SARS-CoV-2.

 

Reference materials: Park, SY, Faraci, G.

, Ward, PMet al.
High-precision and cost-efficient sequencing for real-time COVID-19 surveillance.
Sci Rep 11,13669 (2021).
https://doi.
org/10.
1038/s41598-021-93145-4 

Park, SY, Faraci, G.
, Ward, PM  et al.
 High-precision and cost-efficient sequencing for real-time COVID-19 surveillance.
  Sci Rep 11,  13669 (2021).
https://doi.
org/10.
1038 /s41598-021-93145-4 https://doi.
org/10.
1038/s41598-021-93145-4 Leave a message here