Scientists at East China University have discovered a universal protein cell delivery vector

The Cheng Yiyun team at the School of Life Sciences of East China Normal University has developed and designed a cation polymer rich in benzoic boric acid that enables efficient, universal intracellulation of a variety of proteins and maintains their biological activity without modification. The findings were published recently in Science Advances (2019, 5, eaaw8922).
protein drugs play an important role in biomedicine. Of the top 10 drugs in global pharmaceutical sales in 2018, seven are protein drugs. The development of high-efficiency and safe intracellal delivery of protein is of great significance to the basic research of biomedicine and life science.
, the researchers found that this approach allows boric acid-rich polymers to bind to protein molecules of different isoelectric points and sizes for universal intracellal delivery of proteins. This polymer can deliver more than ten proteins, such as bovine serum albumin, algal hemoglobin, green fluorescent protein, red fluorescent protein, yellow fluorescent protein, cytochrome c, lysosin, trypsin, β-semi-lactosinease, saponin, nuclease, keruclein and so on, and can maintain the bioactive activity of these proteins.
Cheng Yiyun team, in collaboration with Professor Pingyuan of Zhejiang University, evaluated the efficiency of this polymer vector in delivering Cas9 keruclein to a variety of cells and found that it enables efficient CRISPR/Cas9 gene editing.Professor Cheng Yiyun,
, said the polymer has a very high commercial application value and is a universal protein intracellent delivery carrier, and the team is currently advancing the commercial application of the material, a new, more universal protein transfective reagent.
It is known that the first author of the paper is Liu Chongxuan, a doctoral student at the School of Life Sciences of East China University, and Wan Tao, a doctoral student at Zhejiang University, and Professor Cheng Yiyun, a doctoral student at the School of Life Sciences of East China University, and Pingyuan, a professor at Zhejiang University. East China University is the first unit of this thesis. The research has been supported by the National Natural Science Foundation of China's Outstanding Youth Fund, Shanghai's outstanding academic leaders and other projects.
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