Scientists build a system of soy mono-base replacement gene editing techniques.

Original title: Scientists build a soybean mono-base replacement gene editing technology system
Recently, the Chinese Academy of Agricultural Sciences Crop Science Research Institute soybean breeding technology innovation and new varieties breeding innovation team, the use of the modified CRISPR genome editing system, the first to achieve the soybean gene single base replacement, and obtained a stable ideogenic pure mutation system. This study is the first report of soybean mono-base replacement research, which provides new technology and methods for accurately modifying and using single nucleotide polymorphism (SNP) to improve soybean agronomy. The findings were published online in the Journal of Plant Biotechnology.
As a simple and effective genome editing tool, CRISPR has achieved gene-targeted knock-out, allogen mono-base replacement, and endogenic DNA fragment integration in a variety of important crops, but successful practices are rare in soybeans, according to Researcher Hou Wensheng.
The study established a soybean mono-base editing technology system by combining Cas9n inletase, rat cytosine deaminase and uurea glycosylase inhibitors, and successfully realized the single-base directional replacement of the key genes GmFT2a and GmFT4 for soybean flowering regulation, with an efficiency of 18.2% and 6.0%, respectively. The single base substitution of C-T and C-G is realized at the GmFT2a target point, and the single base substitution of C-G is realized at the GmFT4 target. Single-base replacement mutations stabilize heredity, and the resulting GmFT2a single-base replacement pure-lying mutant system exhibits late-flower dedications under 16-hour light/8-hour darkness and 12-hour light/12-hour dark 2 sun conditions. This is the team's first use of CRISPR genome editing system to achieve soybean gene spot knockout, DNA segment deletion, multi-gene mutation, in the application of soybean genome editing technology, further expand the application of soybean genome editing tools and agro-sexual modification means.
research was funded by the National Key Research and Development Program, the National Natural Science Foundation of China and the Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences.
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