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November 27,
published online a joint study by the Institute of Agricultural Environment and Sustainable Development of the Chinese Academy of Agricultural Sciences and the Research Team of the Biotechnology Research Institute. Using magnetic nanoparticles as gene carriers, they created a new method of plant genetic transformation with high flux, convenient operation and wide use, and made important progress in the research of nano-carrier gene transmission and genetic mediatation system, and opened up a new direction of nano-biotechnology research.
the development of high-efficiency and safe new genetic transformation methods has been one of the hot research topics in the fields of genetic engineering, molecular biology and genetic breeding. Traditional plant genetically modified methods usually require complex tissue culture and other plant regeneration procedures in order to obtain genetically modified plants, especially genetically modified plants such as cotton, which are difficult to regenerate crops.
This research and development of magnetic nanoparticle gene carrier-based pollen magnetic transformation plant genetic modification method, can use magnetic nanoparticle Fe3O4 as a carrier, under the guidance of the external magnetic field to the source gene to the pollen, through artificial pollination using the natural reproductive process directly to obtain the conversion seeds, and then after selection to obtain a stable genetically modified offspring.Dr. Zhao Xiang of UNCED, co-first author of the
thesis, told reporters that the method combines nanomagnetic transformation and pollen mediatation to overcome the bottleneck of tissue regeneration culture and host adaptability of traditional genetically modified methods, which can improve the efficiency of genetic transformation, shorten the cultivation cycle of genetically modified plants, achieve high flux and multigene synergy and transformation, have a wide range of applications, are of great significance for accelerating the cultivation of new varieties of genetically modified organisms, and have broad applications in crop genetics, synthesis and biological reactions.
research results have been funded by major special projects of national genetically modified organisms, national "973" project and scientific and technological innovation projects of the Chinese Academy of Agricultural Sciences. The paper's co-first authors are Dr. Wang Wei of the Institute of Environment and Development and Dr. Meng Zhigang of the Institute of Biology, and Cui Hisin, a researcher at the Institute of Environmental Development, and Zhang Rui, a researcher at the Institute of Biology, are co-authors of the communication. (Source: Science Network Li Chen)