Scientists have discovered a new method of genetically modified replacement of plants in place
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Last Update: 2021-03-16
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Source: Internet
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Author: User
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Chen Weiqiang, a postdoctoral fellow at the Center for Molecular Bioanalytics and Genetic Improvement of the South China Botanical Garden of the Chinese Academy of Sciences, and Gurminder Kaur, a former doctoral student, discovered a new method of genetically modified replacement of plants in place under the guidance of researcher Yuxiang. The study was published recently in the Journal of Plant Biotechnology.
In order to solve the difficulties caused by the increase in the number of GM locations scattered in the cultivation of compound genetically modified crops, researchers at the South China Botanical Garden of the Chinese Academy of Sciences discovered a method of GMO spot integration using Bxb1 and Cre recombinant enzymes as early as 2014. This method allows new genotytes to be inserted into existing GM bits, ensuring that all GM can be "packed" and passed on to future generations. However, with the development of the times, the old genetically modified may no longer be needed, how people deal with it has become a problem.
this, the researchers again used the Bxb1 and Cre recombinant enzyme systems to try the replacement of old and new genetically modified. On multigene integration strains with att and lox sequences, a carrier with the new gene OsO3L2-2B and lox bits is first superimposed through the Bxb1-att system, and then the replacement of the old gene cluster gus-gfp-luc and superloads, including the screening marker npt, is completed.
Chen Weiqiang said that since the replacement line also contains att and lox sequences, this means that the superimposing and replacement of new genes can continue when needed. The emergence of this GM replacement system greatly increases the flexibility of multigene molecular breeding, and since the use of Bxb1 and Cre recombinant enzymes is not subject to patent restrictions, it is more conducive to the free use of this breeding technology and greatly improves the possibility of product commercialization. (Source: China Science Journal Zhu Hanbin Zhou fei
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