Scientists in China have succeeded in giving birth to heterozygous twins in a female Buffalo

Introduction: using advanced biotechnology, Chinese scientists have successfully produced two calves of different breeds from one cow Under natural conditions, buffalo have only two out of 100000 twins The success of this technology greatly increases the possibility of obtaining twin Buffalo, which is conducive to the rapid expansion of high-quality breeding cattle According to Huang Youjun, a researcher at Guangxi Buffalo Research Institute, a female buffalo who received artificial fertilization and embryo transfer successively gave birth to twins in the Research Institute on the evening of the 22nd day after 300 days of normal pregnancy The two calves were pure Neri Raffi cattle and hybrid Mola buffalo Among them, the weight of nerirafi is 35 kg, and the weight of hybrid Mora is 31 kg Two calves can walk and milk soon after landing Previously, there had been no reports of the birth of buffalo heterozygous twins in the world Nirirafi and mora buffaloes originated from Pakistan and India are milk buffaloes, which are being used in southern China to develop milk industry Huang Youjun said that in October last year, his team used artificial fertilization to put the sperm of hybrid Mola cattle into the cow's body; then, using embryo transfer technology, put the blastocyst of a Neri Lafi cattle into the same cow's body, so that it can conceive an alien twin Before blastocyst transplantation, the researchers first cultured and fertilized the ovary oocytes of nilirafei cattle in vitro, and then the fertilized eggs were cultured to become the blastocyst Another difficulty of this technique is to culture buffalo oocytes into blastocysts Because of the poor quality of buffalo oocytes, sperm capacitation in vitro is low.