echemi logo
  • Product
  • Supplier
  • Inquiry
    Home > Food News > Food Articles > Scientists reveal single-cell heterogeneity and differentiation of rice roots

    Scientists reveal single-cell heterogeneity and differentiation of rice roots

    • Last Update: 2021-04-17
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit

    Scientists reveal single-cell heterogeneity and differentiation of rice roots

    Scientists Reveal the Panorama of Rice Root Single Cell Heterogeneity and Differentiation Scientists Reveal the Panorama of Rice Root Single Cell Heterogeneity and Differentiation


    The research group of Wang Jiawei, Center for Excellence in Molecular Plant Science, Chinese Academy of Sciences, systematically revealed the heterogeneity of rice root single cells, described the differentiation trajectory of rice root epidermal cells and basic tissue cells, and clarified gene expression and gene staining during root tip stem cell differentiation.
    The relevance of quality accessibility, and at the same time clarified the evolutionary conservation of the monocotyledonous plant rice and the dicotyledonous plant Arabidopsis in the root tip cell types.
    The research results were published online in Nature-Communication on April 6.

    Rice is one of the important food crops.
    Roots are important tissues and organs of rice, responsible for fixing and supporting plants, absorbing water and mineral elements from the soil to supply plants for growth and development, and participating in the interaction between plants and biological or non-biological signals.
    Different from the dicotyledonous model plant Arabidopsis thaliana, rice is a fibrous root plant with different growth and development patterns.
    In addition, in order to better adapt to the aquatic environment, rice has also evolved specialized structures such as cortical tissue, thick-walled tissue, aerated tissue, and multicellular cortical tissue.
    Genetic mutant screening is a powerful method for traditional developmental biology and functional genomics to obtain important regulatory genes, but it is often limited by unfavorable factors such as long research cycle, black box testing, quantitative traits, and heavy workload.
    In recent years, with the invention and application of single-cell RNA sequencing technology, researchers can systematically understand the heterogeneity of living cells at the single-cell level, depict the differentiation and development trajectory of various types of cells, and accelerate the mining of important regulatory factors during development process.

    The previous work of Wang Jiawei's research group described the root single-cell map of the dicotyledonous model plant Arabidopsis thaliana.
    In order to further explore the developmental patterns of monocotyledonous plant roots and reveal the evolutionary laws of monocotyledonous plant roots, the researchers applied scRNA-seq and ATAC-seq technologies to the study of rice roots.
    The scRNA-seq experiment successfully captured 27469 high-quality single-cell transcriptome data.
    Through cluster analysis, these cells were annotated into 21 different cell groups, corresponding to rice root epidermis, outer cortex, thick-walled tissue, cortex, endothelial layer, pericyll, meristem, vascular tissue and other cell groups.
    Through in situ hybridization and construction of reporter genes, a series of brand-new cell type marker genes were discovered and verified.
    Further through the rearrangement of transition state cells and pseudo-time analysis, the development process of epidermal meristem cells forming trichome cells or non-trichome cells through division and differentiation was described, and the differentiation of basic meristem progenitor cells to form cortex and thick wall was clarified.
    Differentiation trajectory of tissue and outer cortex.

    Interestingly, the integration analysis of scRNA-seq and ATAC-seq shows that the chromatin open state of some important regulatory factors is temporally and spatially related to their gene expression patterns.
    More importantly, through a combination of reverse genetics experiments, it was found that the transcription factor OsGATA6, which is specifically expressed in rice root meristems, is involved in the development of rice root basic tissues and vascular bundle tissues, suggesting that single-cell sequencing technology can effectively improve crop response.
    To the success efficiency of genetics.

    At the same time, the researchers analyzed with the Arabidopsis root single-cell transcriptome data set and found that the two cell types are less conservative in evolution.
    Only in root hair, xylem, phloem and other cell types, there are high similarities.
    Further in-depth comparison and analysis of these conserved cell types, unearthed some potential core cell type regulatory genes.

    Experts said that these research results helped us draw a single-cell map of rice roots, laying a good foundation for the future analysis of the fine process and molecular mechanism of rice root development, artificially customizing the root system, and improving nutrient absorption capacity.
    (Source: Huang Xin, China Science News)

    Related paper information: org/10.
    1038/s41467-021-22352-4" target="_blank">https://doi.

    1038/s41467-021-22352-4" target="_blank">https://doi.
    This article is an English version of an article which is originally in the Chinese language on and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.
    Related Articles

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent Echemi's opinion. If you have any queries, please write to It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to with relevant evidence.