Screening Chemical Libraries for Compounds That Affect Protein Sorting to the Yeast Vacuole
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Last Update: 2021-01-17
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Source: Internet
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Author: User
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Protein trafficking to the yeast vacuole has been extensively studied using a series of deletion mutants. In these genetic screens, mis-targeted vacuolar cargo proteins were used as phenotype markers. Here we describe a similar approach employing pharmacological effects of diverse chemical compounds to mimic molecular phenotypes caused by conventional genetic mutations in protein trafficking genes.
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