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    Home > Biochemistry News > Plant Extracts News > Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview

    Selected Reaction Monitoring Mass Spectrometry: A Methodology Overview

    • Last Update: 2020-11-10
    • Source: Internet
    • Author: User
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    Moving past the discovery phase of proteomics, the term targeted proteomics combines multiple approaches investigating a certain set of proteins in more detail. One such targeted proteomics approach is the combination of liquid chromatography and selected or multiple reaction monitoring mass spectrometry (SRM, MRM). SRM-MS requires prior knowledge of the fragmentation pattern of peptides, as the presence of the analyte in a sample is determined by measuring the
    m/z
    values of predefined precursor and fragment ions. Using scheduled SRM-MS, many analytes can robustly be monitored allowing for high-throughput sample analysis of the same set of proteins over many conditions. In this chapter, fundaments of SRM-MS are explained as well as an optimized SRM pipeline from assay generation to data analyzed.
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