Selection and use of amino acid columns.

: My experiment needs to use HPLC to do a variety of
amino acids
solution quantitative analysis, please ask in the use of amino acid column, is it as long as direct sample, do not need to do a different amino acid diffraction
national
?
ask a question, in the selection of amino acid column generally choose which model is better, I mainly test 8 essential amino acids, amino acid column and HPLC models to match? I am a super newman, please know the friends to help, thank you very much!
: Analysis of amino acids must be derived, because the vast majority of amino acids are not UV absorption, only a few amino acids containing benzene ring can be significantly absorbed, that is, the vast majority of amino acids in the liquid phase most commonly used ultraviolet detector no signal response, of course, it is not possible to quantitative analysis. You measure 8 must amino acids more to be derived treatment.
As for what kind of column to choose, the major
chromatography
column manufacturers have specifically for amino acid analysis and the introduction of columns, the analysis effect is good, choose which problem is not big, you can choose Angelen's Eclipse AAA. There is also no amino acid column and HPLC model with this said, any brand column can be used in any brand of HPLC system.
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