Separation and determination of ferulic acid in Angelica by capillary zone electrophoresis

Key words: capillary zone electrophoresis; ferulic acid; Angelica high performance capillary electrophoresis (HPCE) is a new separation and analysis technology developed rapidly in recent ten years, which has the characteristics of high education, high speed, low consumption and small sample consumption Capillary zone electrophoresis (CZE) is a separation mode of HPCE It is based on the difference of electrophoretic mobility of charged components in the external electric field, and has been widely used in the analysis of traditional Chinese medicine Angelica sinensis is one of the commonly used traditional Chinese medicines in clinical Zui Ferulic acid is one of the main effective components to inhibit platelet aggregation Therefore, thin-layer scanning method and liquid phase method are often used to determine the content of ferulic acid, which is one of the indexes to evaluate the quality of Angelica sinensis and its preparations The content of ferulic acid in Angelica sinensis was determined by CZE method with satisfactory results 1 Materials and methods 1 μ m × 100cm Ferulic acid and diclofenac standard reference were provided by the Chinese pharmaceutical and biological product orange Ding Angelica is sold on the market Borax and hexanol are analytical pure 1.2 the buffer solution of electrophoresis condition is 20 mmol / L borax solution, the operating voltage is 25 kV, the temperature is 25 ℃, the sample is siphoned, the time is 10 s, the UV detection on the column, the wavelength is 214 nm 1.3 for sample determination, accurately weigh proper amount of Angelica sinensis powder, place it in a 25ml volumetric flask, add appropriate amount of 70% ethanol, and extract it by ultrasonic vibration for 45min The concentration of diclofenac was 40 μ g / ml, and the supernatant was centrifuged for determination 1.4 accurately weigh appropriate amount of Angelica sinensis fine powder and place it in a 25ml volumetric flask Add appropriate amount of ferulic acid reference, operate according to the steps under sample determination and calculate the recovery rate Results and discussion 2.1 the Zui absorption wavelength of ferulic acid is about 313 nm However, due to the limitation of the instrument conditions, we use 214nm to detect ferulic acid, which still has good results In this experiment, the preparation buffer was used for separation Through the effect of preparation concentration on Danli, 20 mmol / L borax solution was determined as the preparation buffer Under this condition, the other components of Angelica sinensis were separated well from ferulic acid and the selected internal standard 2.2 take 10, 2O, 40, 60, 80 μ g of ferulic acid reference substance from the standard curve and put them into a 1ml volumetric flask, respectively, add 40 μ g of internal standard, and fix the volume with 70% ethanol The ratio of the peak area of ferulic acid to the internal standard was recorded The regression equation between Y and X was y = 30.12x-0.02076 (r = 0.9992) 2.3 the precision of ferulic acid was tested by three concentrations of high, medium and low in the day and in the day The results showed that the determination method was rational 2.4 recovery of ferulic acid was 97.25%, RSD was 1.94% (n = 5) 2.5 the established method was used to determine 3 batches of Angelica sinensis for 5 times The results showed that the content of ferulic acid in Angelica sinensis was quite different, which was related to the different origin or harvest season of Angelica sinensis, and also showed the necessity of quality control of Angelica sinensis Conclusion the CZE method with good linear relationship and reproducibility was established for the separation and determination of ferulic acid in Angelica The method is accurate and simple It provides a new method for the quality control of Angelica It can be predicted that the characteristics of CZE method will make it have a very attractive prospect in the analysis of traditional Chinese medicine Reference 1: Liu Zhisong, Fang zhaolun, application of capillary electrophoresis in drug analysis [J] Chromatography, 1996, 14 (5): 364