Shanghai Jiaotong University Yang Litao team developed a new method Cas-PfLAMP for rapid field detection of rice pathogens

Recently, Yang Litao's research group from Zhang Dabing's team from Shanghai Jiaotong University's School of Life Science and Technology published a paper entitled "PAM-free Loop-mediated Isothermal Amplification Coupled with CRISPR/ Cas12a Cleavage (Cas-PfLAMP) for Rapid Detection of Rice Pathogens" research paper, which for the first time combined nucleic acid solid-phase extraction, LAMP isothermal amplification, CRISPR/Cas12a in vitro shearing and immunoassay strips to establish an on-site rapid nucleic acid detection The technical system Cas-PfLAMP has been successfully applied to the rapid detection of field rice pathogens (rice bacterial blight, XOO; rice stripe virus disease, RSV; rice black-streaked dwarf disease, RBSDV)

Rice (Oryza sativa L.

In recent years, the clustered regularly interspaced short palindromic repeats (Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR/Cas) system has been widely used in the field of genome editing and nucleic acid detection

In this study, the authors developed a loop-mediated isothermal amplification (LAMP) detection technology that does not rely on spacer adjacent motif (PAM) and CRISPR/FnCas12a splicing-assisted, Cas-PfLAMP

Schematic diagram of the working principle of Cas-PfLAMP

At the same time, the researchers also invented a new method for solid-phase extraction of nucleic acid from plant leaves.

Schematic diagram of the workflow of the Cas-PfLAMP field rapid visualization detection platform

The research was greatly assisted by Professor Chen Gongyou from the School of Agriculture and Biotechnology of Shanghai Jiao Tong University, and Zhou Tong, a researcher from Jiangsu Academy of Agricultural Sciences.

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