Shanghai Jiaotong University Zhang Yu's team designed new primers to help detect microbial diversity in trenches

Recently, Yang Na, a doctoral student under the guidance of researcher Zhang Yu from the School of Oceanography, Shanghai Jiao Tong University, published a paper entitled "Novel primers for 16S rRNA gene-based archaeal and bacterial community analysis in oceanic trench sediments" in the classic journal "Applied Microbiology and Biotechnology" in the field of environmental microbiology.

Trench is a steep, long and narrow trench located on the edge of the deep-sea plate, with ultra-high hydrostatic pressure and relatively isolated hydrogeology, which nurtures a unique microbial ecosystem

Newly designed primers detect more rare archaeal and bacterial taxa in Mariana Trench sediment samples than conventional universal primers

In this study, based on the clone library technology, the researchers constructed 396 archaeal and 1868 bacterial 16S rRNA gene full-length sequences using sediment samples from the Mariana Trench, respectively

The study found that, compared with the universal primers, in 30 Mariana Trench sediments, 46 family-level bacteria could only be detected by the newly designed primers B344F/B749R; 8 family-level archaea could only be detected by the newly designed primers B344F/B749R.

Paper link : https://link.