-
Categories
-
Pharmaceutical Intermediates
-
Active Pharmaceutical Ingredients
-
Food Additives
- Industrial Coatings
- Agrochemicals
- Dyes and Pigments
- Surfactant
- Flavors and Fragrances
- Chemical Reagents
- Catalyst and Auxiliary
- Natural Products
- Inorganic Chemistry
-
Organic Chemistry
-
Biochemical Engineering
- Analytical Chemistry
- Cosmetic Ingredient
-
Pharmaceutical Intermediates
Promotion
ECHEMI Mall
Wholesale
Weekly Price
Exhibition
News
-
Trade Service
Recently, the team of Zheng Wei, a researcher at the Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, proposed a fast three-dimensional imaging technology based on excitation light gradient encoding, which can increase the speed of two-photon volume imaging by 5 to 10 times compared to traditional techniques
.
With submicron imaging resolution and millimeter imaging depth, two-photon microscopy is widely used in neural structural and functional imaging and other in vivo imaging studies
.
Traditional two-photon three-dimensional imaging is achieved by scanning the focus of two-photon excitation in the sample layer-by-layer two-dimensional scanning.
The new gradient light field two-photon microscopy imaging technology proposed in this study only needs two two-dimensional scans to obtain three-dimensional information of the sample, which greatly reduces the damage of the laser to the sample
.
In real life, you can use coding to determine location
.
Similarly, gradient light field technology designs a pair of axially elongated foci with varying intensity gradients, and uses the intensity changes of the pair of foci to encode and resolve the position of the object: in the image obtained by scanning the first gradient focus laterally , the fluorescence intensity of the sample in the shallow position is strong, and the fluorescence intensity of the sample in the deep position is weak, and the image corresponding to the second focus is just the opposite
The related results were published in Photonics Research under the title of Axial gradient excitation accelerates volumetric imaging of two-photon microscopy
.
The research is supported by the National Natural Science Foundation of China's major scientific research instrument development projects, major research plans, and Guangdong Provincial Key Laboratory
(a): Gradient light field two-photon microscopy imaging principle, (b): Macrophage phagocytosis process, (c): Ball trajectory, (d): Quantification and evaluation of the ball trajectory
