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Through genome sequencing, a single cell can reveal a lot of information about the biological world
However, despite the rapid development of single-cell sequencing technology, sample preparation is still far behind, and it has increasingly become the main bottleneck hindering the widespread application of single-cell technology
To solve this problem, researchers at the Qingdao Institute of Bioenergy and Process Technology (QIBEBT) of the Chinese Academy of Sciences combined single-cell sample preparation and sequencing methods into a single, streamlined method called Positionable Dynamic Droplet Array (aDDA) )
Their research was published in the journal Small on July 23
“Existing single-cell sequencing methods usually cannot track a specific cell, from sample preparation to sequencing, which obscures the results of how the genome matches the specific function of the corresponding cell
"The ideal droplet microfluidic platform for sample preparation requires not only static characteristics, such as accurate identification and retrieval of single single-cell droplets, but also the ability to precisely control biochemical reactions on a pico-liter scale.
The static droplet array enables researchers to accurately identify and retrieve droplets containing target single cells, while the continuous flow droplet platform can process each step continuously and quickly
To validate their method, the researchers processed the genome of a single yeast cell
"Currently, this platform is limited by throughput because the retrieval steps are still manual," said MA Bo, deputy director of the Single Cell Center and senior author of this study, noting that the team is working to automate the retrieval process
Original search: Small.