echemi logo
Product
  • Product
  • Supplier
  • Inquiry
    Home > Biochemistry News > Biotechnology News > Simultaneous Determination of Atenolol and Amiloride by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection (C4D)

    Simultaneous Determination of Atenolol and Amiloride by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection (C4D)

    • Last Update: 2021-01-02
    • Source: Internet
    • Author: User
    Search more information of high quality chemicals, good prices and reliable suppliers, visit www.echemi.com
    Capillary electrophoresis coupled with a capacitively coupled contactless conductivity detector (CE-C
    4
    D) has been employed for the determination of the β-blocker drugs (atenolol and amiloride) in pharmaceutical formulations.150 mM acetic acid was used as background electrolyte. The influence of several factors (detector excitation voltage and frequency,buffer concentration, applied voltage, capillary temperature, and injection time) was studied. Non-UV absorbing
    l
    -valine was used as an internal standard; the analytes were all separated in less than 7 min. The separation was carried outin normal polarity mode at 28 �C, 25 kV, and using hydrodynamic injection (25 s). The separation was effected in a bare fused-silicacapillary 75 μm � 52 cm. The CE-C
    4
    D method was validated with respect to linearity, limit of detection and quantification, accuracy, precision, and selectivity. Calibration curves were linear over the range 5–250 μg mL
    −1
    for the studied analytes. The relative standard deviations of intra- and inter-day precisions of migration times and correctedpeak areas were less than 6.0%. The method showed good precision and accuracy and was successfully applied to the simultaneousdetermination of the β-blocker drugs in different pharmaceutical tablets.
    This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only. This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed description of the concern or complaint, to service@echemi.com. A staff member will contact you within 5 working days. Once verified, infringing content will be removed immediately.

    Contact Us

    The source of this page with content of products and services is from Internet, which doesn't represent ECHEMI's opinion. If you have any queries, please write to service@echemi.com. It will be replied within 5 days.

    Moreover, if you find any instances of plagiarism from the page, please send email to service@echemi.com with relevant evidence.