Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis
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Last Update: 2021-02-24
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Source: Internet
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Author: User
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The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize
DNA
damage in individual cells (
1
). The method was modified by adding strong alkaline conditions to denature genomic DNA and detect alkali labile lesions, greatly improving sensitivity for single-strand breaks (
2
,
3
). This simple assay has come into widespread use because of its sensitivity, need for few cells, applicability to nearly all eukaryotic cells, and ease in processing numerous samples (
4
).
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