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As a tumor optical therapy strategy, photothermal therapy can kill cancer cells locally in a targeted manner, and has the characteristics of minimally invasive, long-acting and safe in the treatment of malignant tumors.
However, many photothermal therapy agents are not ideal due to poor biocompatibility, complex reaction methods in the production and processing process, and harsh reaction conditions
.
Therefore, the development of photothermal agents with good biocompatibility under environmentally friendly conditions is of great research significance
.
As a multifunctional pigment, melanin is widely used in energy and biomedical fields, especially in cancer imaging diagnosis and photothermal therapy
.
At present, the melanin on the market is mainly obtained by chemical synthesis or extraction from squid, and these methods are not suitable for large-scale production
.
In contrast, biosynthetic melanin has the advantages of mild reaction conditions, environmental friendliness and good biocompatibility.
Recently, the team of Yan Fei, a researcher at the Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, polymerized to generate melanin nanoparticles by biosynthesis, and the related research results were based on "Biosynthesis of Melanin Nanoparticles for Photoacoustic Imaging Guided Photothermal.
" Therapy" was published in Small
, an internationally renowned journal in the field of materials.
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Article link
The research team expressed tyrosinase heterologous in E.
coli and catalyzed the hydroxylation of substrate L-tyrosine to 3,4- Dihydroxyphenylalanine (L-DOPA), L-DOPA further oxidizes to form dopaquinone, and dopaquinone polymerizes to form melanin nanoparticles
.
In addition, the research team characterized the structure of the biosynthesized melanin nanoparticles, evaluating their photothermal performance and biological safety
.
The experimental results show that the biosynthesized melanin nanoparticles have good photothermal stability, high photothermal conversion efficiency, good biocompatibility, excellent photoacoustic imaging performance, and can be used as an environmentally friendly photothermal therapy agent for photoacoustic imaging-guided photothermal therapy Important research value and application prospects
.
Figure 1 Schematic diagram of biosynthetic melanin nanoparticles for photoacoustic imaging-guided photothermal therapy for tumors
Figure 2 Biosynthesis and characterization
of melanin nanoparticles.
(a) Schematic diagram of melanin nanoparticle biosynthesis in E.
coli, (b).
Representative pictures at different time points during melanin biosynthesis, (c) quantitative analysis of the yield of melanin production at different time points (d) Samples from different time points (0, 3 h, 6 h, 12 h) during melanin nanoparticle biosynthesis were constructed and sequenced for analysis (e) Volcano map expressing significantly altered genes, (f) TEM map of melanin nanoparticles (Scale bar =50 nm), (g)DLS determination of melanin nanoparticles Hydration dynamic diameter, (h) UV-visible absorption spectra of biosynthetic melanin nanoparticles at different concentrations.
Shenzhen Advanced Institute is the first communication
.
Yan Fei, researcher of Shenzhen Advanced Hospital, Professor Lin Jing of Shenzhen University, and Xu Xiaohong, chief physician of the Affiliated Hospital of Guangdong Medical University, are the co-corresponding authors
of the article.
Fu Meijun, a doctoral candidate from Shenzhen Advanced Institute, Yang Yuping, a doctoral student at Southern Medical University, and Zhang Zhaomeng, a master's student at Hebei Medical University, are the co-first authors
of the article.
The research work has been supported
by the Key R&D Program of the Ministry of Science and Technology of the People's Republic of China, the General Project of the National Natural Science Foundation of China, the Shenzhen Science and Technology Innovation Commission, and the Shenzhen Innovation Institute of Synthetic Biology.
