Soybean: Gmax_ZH13 gene annotation gene to build a complete gene co-expression network.

Soybean is an important food cash crop, which provides the main oil and protein resources for human beings.
soybeanoriginat originated in China, the ancient name "yu", was domesticated by its wild species about 5,000 years ago, and then spread widely around the world.
soybeans have a genetic bottleneck effect in the process of introduction and improvement, which makes significant genetic variation among soybean varieties from different main producing areas.
currently, our widely used soy reference genome is derived from the U.S. variety "Williams 82" (Glycine_max_v2.0).
the genome of this single species does not fully represent the genetic variation of all soybeans, especially Asian species with significant genetic variations at a distance from the United States geographical distance.
in addition, the functional study found that there were many assembly errors in the genome, which affected the location mining of functional genes.
, the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, in conjunction with the China University of Science and Technology, the Institute of Seed Resources and Biotechnology of the Jiangsu Academy of Agricultural Sciences, Beijing Berry and Kang Biotechnology Co., Ltd., combined with single-molecule real-time sequencing (SMRT), single-molecule optical mapping (single-molecule optical) and high-throughput chromosomal conformation capture technology (Hi-C), the Chinese national soybean varieties "middle yellow 13" genome (Gmax_ZH13) from the head assembly, and finally obtained 1.025 gb of genome sequence.
the genome Contig N50 is 3.46 Mb and The Scaffold N50 is 51.87 Mb, one of the best continuous plant genomes available. Further analysis
showed that there were a large number of genetic variations between the Gmax_ZH13 and Williams 82 genomes, including 1404 translocationevents, 161 inverted events, 1233 inverted transposition events, and 505,506 small inserts/missing (1-99 bp) and 17,409 large inserts/missings (100 bp) in Gmax_ZH13.
the study combined a large amount of transcription data to build a complete gene co-expression network for Gmax_ZH13 gene annotation genes.
through the co-expression relationship between the genes that have been reported to control soybean flowering time and the candidate genes in the newly positioned QTL or GWAS interval, the gene that controls the traits in the new positioning interval is more precisely screened, 26 genes that may control soybean flowering time are obtained, and some of the genes are verified by the association of natural group genetic variation and phenotype differences, which provides a new way of mining important agronomic genes. The publication of
Gmax_ZH13 genome provides an important resource for soybean basic research and lays the foundation for the cultivation of excellent soybean varieties in China.
research was published online July 27 in the form of a cover article in Science China Life Science, entitled De Novo Assembly of a Chinese Seabe. Tian Zhixi, a researcher at the Institute of Genetic Development of
, is the first author of the paper, Tian Zhixi is the first author of the paper, Ma Shixuan, a professor at the University of Science and Technology of China, and Du Jianfactor, a researcher at the Institute of Seed Resources and Biotechnology of Jiangsu Academy of Agricultural Sciences, are co-authors.
the research was funded by the National Science Foundation of China, the National Key Laboratory Project for Strategic Pilot Science and Technology (Class A), Plant Cells and Chromosomal Engineering.
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