Staining of Preparative 2-D Gels: Coomassie Blue and Imidazole-Zinc Negative Staining
Last Update: 2021-02-28
Search more information of high quality chemicals, good prices and reliable suppliers, visit
The identification of proteins using preparative gel electrophoresis and mass spectrometry requires reversible staining of relatively thick (1–1.5 mm) polyacrylamide gels. We have found that staining with colloidal Coomassie brilliant blue G-250 or negative staining with imidazole-zinc yields high-resolution stains (Fig. 1 ) that are compatible with subsequent mass spectrometric analysis (
Notes 1 and 5 ).
Preparative 2-D gels stained by the imidazole-zinc negative stain (left) and colloidal Coomassie blue G-250 stain (right). Gels were scanned by a Computing Densitometer (Molecular Dynamics, CA). Top: 1 mg of protein from ME-180 cervical carcinoma cells was separated by carrier ampholyte IEF and 11%
-PAGE, and then stained using the imidazole-zinc stain. Bottom: 1 mg of protein from A375 human melanoma cells was separated by carrier ampholyte IEF and 11% SDS-PAGE, and then stained using the colloidal Coomassie blue G-250.
This article is an English version of an article which is originally in the Chinese language on echemi.com and is provided for information purposes only.
This website makes no representation or warranty of any kind, either expressed or implied, as to the accuracy, completeness ownership or reliability of
the article or any translations thereof. If you have any concerns or complaints relating to the article, please send an email, providing a detailed
description of the concern or complaint, to firstname.lastname@example.org
. A staff member will contact you within 5 working days. Once verified, infringing content
will be removed immediately.