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    Home > Active Ingredient News > Drugs Articles > Standard curve in element analyzer

    Standard curve in element analyzer

    • Last Update: 2013-05-27
    • Source: Internet
    • Author: User
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    The standard curve in the element analyzer refers to the relationship between a series of substances with known content (concentration / quantity) and the response / signal of the instrument The mathematical processing is the curve equation, and the graphic representation is the standard curve in the coordinate The purpose of the standard curve is to find out the content of the substance to be tested according to the standard curve When we get the response of a series of substances with known content, we will establish the function relationship, which is called curve fitting mathematically Because the straight line Zui is simple, we often use the straight line equation to fit, of course, we will use polynomial fitting and other methods The core problem of the standard curve should be solved: 1 It can find the standard substance or standard substance with exact concentration 2 The standard series and the substance to be tested must have the same and consistent matrix, because the matrix of the sample may interfere with the response of the instrument In this sense, the pretreatment of the sample is actually to provide the same matrix environment of the standard and the sample, and try to eliminate the interference matrix So a good standard series of Zui should be the standard series of sample matrix matching In the process of establishing the method of ore analyzer, the first thing to be considered is the matrix interference It is recommended to use the standard addition curve and Youden curve to investigate the product interference and additive interference brought by the sample matrix respectively The standard addition curve is to add a series of standards to the sample, and then investigate the statistical difference between the standard addition curve and the slope of the standard curve If there is any difference, the standard addition method should be considered for quantification; while the Youden curve is to make a series of dilutions to the sample, and then use the dilution times such as 1 / 10, 1 / 5, 1 / 2, 1 Make a curve for the response of the instrument, and investigate the difference between the intercept of the Youden curve and 0 If there is a difference, it indicates that there is additive interference At this time, the measured value should be subtracted from the intercept to be the real value Only by solving the matching problem between the standard curve and the sample matrix, can our quantification be reliable The use of internal standard method and substitute is to solve the influence of element analysis instrument and pretreatment.
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