Standard Operating Procedure for Biological Safety Cabinets

Notes on Standard Operating Procedures 1.
The safety cabinet should be placed in the primary clean room below 100,000 class
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Before the first operation every day, turn on the UV lamp and irradiate it for 30 minutes, then move all the items required for this operation into the safety cabinet to avoid frequent passage of the arms through the air curtain to destroy the airflow; and wipe the surface of the items with 75% alcohol to disinfect before moving in.
to remove contamination
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2.
Turn on the fan for 10 minutes, wait until the air in the cabinet is purified and the airflow is stable before performing the experimental operation
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Slowly extend your arms into the safety cabinet and keep it still for at least 2 minutes to stabilize the airflow in the cabinet before proceeding
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3.
Items unrelated to this experiment should not be placed in the biological safety cabinet.
The items required for the experiment should be kept as simple as possible.
Avoid covering the grille of the safety cabinet with items to minimize the impact on airflow
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Items should be placed as far back as possible without blocking the airway opening, so as not to interfere with the normal flow of airflow
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4.
Operation should avoid cross-contamination
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To prevent possible spills, have a 75% alcohol spray can and paper towels ready to wipe down the droplets as soon as possible
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5.
During the experimental operation, the glass window should not be fully opened, and the operator's face should be kept above the working window
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When operating in the cabinet, the movements should be gentle and soothing to prevent affecting the airflow in the cabinet
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6.
The safety cabinet should be inspected and maintained regularly to ensure its normal operation
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Once the safety cabinet is found to be abnormal during work, stop work immediately, take corresponding measures, and notify the instrument manager
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7.
After the experiment is completed, close the glass window, keep the fan running for 10 minutes, and turn on the UV lamp for 30 minutes.

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8.
The safety cabinet should be cleaned and disinfected regularly, and 75% alcohol can be used to wipe the work surface and the outer surface of the cabinet; it should be fully disinfected after each inspection
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9.
Items used in the cabinet should be sprayed with 75% ethanol to disinfect the surface before taking them out to prevent potential pathogen residues from being brought out to pollute the environment and cause biological hazards
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 How to use: 1.
The safety cabinet is controlled by LED liquid crystal panel.
The control panel includes function keys: power switch key, germicidal lamp key, windshield key, and lighting key
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 2.
After connecting the 220V AC power supply, press the power switch shown on the control panel to turn it on; when sterilization is required, press the sterilization lamp key; and turn on the fan, according to the situation; key
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 Equipment maintenance 1.
Use 75% alcohol to thoroughly decontaminate the surface, side walls, rear walls and windows of the inner working area of ​​the safety cabinet after each inspection operation
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Never use disinfectants containing chlorine as it may damage the stainless steel construction of the cabinet
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At the same time, clean the surface of the UV lamp and the power outlet
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2.
Cleaning and maintenance must be carried out every two weeks when there is no experiment for a long time.
Follow the above plan.
Regular cleaning of the stainless steel surface will keep the surface smooth and beautiful
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  3.
Wipe the outer surface of the safety cabinet with a damp cloth every month, especially the front and upper parts of the safety cabinet, to clean up the accumulated dust
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And check the reasonable use of all maintenance accessories
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  4.
According to the actual situation, check the safety cabinet for any physical abnormality or failure every quarter or half a year, and report for repair in time if there is any abnormality; remove the primary filter for cleaning to prevent dust accumulation, which will lead to insufficient air intake and reduce the cleaning effect
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  5.
Ask qualified certified technicians to perform performance certification of the safety cabinet every year, and replace the UV lamp according to the service life effect of the UV lamp; after normal adjustment or cleaning of the primary air filter, the ideal cross-sectional wind speed is still not achieved.
When the fan is turned on, the working voltage of the fan should be adjusted (rotate the knob) to achieve the ideal uniform wind speed (adjust the wind speed knob slowly from left to right, from low speed to high speed, and the new workbench should not be adjusted to the highest wind speed)
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  6.
After 18 months of use, when the working voltage of the fan is adjusted to the highest point, and the ideal wind speed is still not achieved, it means that the high efficiency air filter has too much dust (the filter holes on the filter material have been basically blocked, and should be updated in time.
), the general service life of the high-efficiency air filter is 18 months; when replacing the high-efficiency air filter, you should pay attention to the model size (configured by the original manufacturer), install it according to the arrow wind direction, and pay attention to the surrounding sealing of the filter, there is absolutely no Leakage occurs
.