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    Home > Chemicals Industry > Chemical Technology > Steroid Anabolic Hormone Drug Residue Analysis Technology-Pretreatment Method (3)

    Steroid Anabolic Hormone Drug Residue Analysis Technology-Pretreatment Method (3)

    • Last Update: 2021-09-20
    • Source: Internet
    • Author: User
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    3) Immunoaffinity chromatography (IAC)

    The principle of IAC is to immobilize the antibody on the solid-phase carrier material to make an immunoaffinity adsorbent, pass the sample solution through the adsorbent, and the target compound in the sample is retained on the solid-phase adsorbent due to the immunoaffinity interaction with the antibody On
    .


    Then, a buffer or an organic solvent is used as an eluent to dissociate the target compound from the antibody, so that the target compound is extracted and purified


    Xu Yan et al.
    coupled the polyclonal antibody to dehydromethyltestosterone with CNBr-Sepharose 4B to make an IAC column with specific immunological adsorption to dehydromethyltestosterone, and optimized the preparation and use conditions; at the same time, using indirect The performance of the IAC column was evaluated by competitive enzyme-linked immunosorbent (ELISA) and HPLC methods
    .


    The optimal reaction time of CNBr-Sepharose4B conjugated antibody is 2.


    4) Gel permeation chromatography (GPC)

    GPC is separated according to the relative molecular mass or volume of the analyte.
    The larger relative molecular mass flows out first, and the smaller relative molecular mass flows out later.
    It is suitable for the purification of small molecular drugs in large molecular substances such as fats
    .


    Kaklamanos et al.


    5) Matrix solid phase dispersion (MSPD)

    MSPD is a fast, simple, cheap, efficient and durable sample processing technology.
    Its basic operation is to mix and grind the sample directly with an appropriate amount of reversed-phase bonded silica, so that the sample is uniformly dispersed on the surface of the stationary phase particles.
    The semi-solid is loaded into a chromatography column, and then washed and eluted using an operation similar to SPE
    .


    MSPD avoids the loss of the analyte caused by sample homogenization, dissolution, emulsification, and concentration


    6) turbulent flow chromatography (TFC)

    The vortex chromatography technology uses large-particle fillers to generate a vortex state in the mobile phase at a high flow rate, thereby purifying and enriching biological samples
    .


    Now it has developed into a pre-processing technology for direct sample injection, rapid purification and separation of biological samples


    Related Links: Analytical Technology of Steroid Anabolic Hormone Drug Residues-Pretreatment Method (2)

     

     

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