Sticky end connection.

. NameIf the end sequence has the same restrictive
nucleic acid
internal shea-cutting enzyme bits as on the vector, then the same limit enzyme cut-off produced by the sticky end, in reducing the temperature of apocalypse, can be re-complementary binding, in
DNA
connecting enzyme catalysis, the destination sequence is connected to the carrier DNA chain (see Figure 20-5).
20-5 the same restriction enzyme cuts the connection at the sticky end of dna . Different
restrictive endoenzymes
cheted, if the resulting DNA has the same sticky end, can also be connected by this method, such as identifying the 6bp sequence of BamHI. and identifying the 4bp sequence of Sau3aI. after cutting dna to produce 5' protruding viscous end GATC, can complement the binding connection.If the two connected DNA fragments do not have a viscous end that complements each other, the end-turbulence
nucleotides
transmostic enzymes
catalytic deamination mononucleotides can be used to add 3' ends of DNA, e.g. general DNA 3' ends Add polyG, another strand of DNA plus polyC, so that artificially at both ends of the DNA can complement the conucleotide polypolymer viscous end, de-inflammation can be combined and connected (Figure 20-6), such a method is called the copolymer tailing method.
figure 20-6 with the polygam and tailings even rafa . For the flat end of the DNA, can also be first connected to the synthetic deoxybonized oligonucleotide double link head, so that the end of the DNA to produce a new limiting endoenzyme site, after the end-cutting enzyme, can be connected by the sticky end (Figure 20-7).
20-7 manual connector connection method is

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