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Strain activation is to put the preserved strains in a suitable medium for culturing, and gradually expand the culture to obtain a pure and strong culture, that is, to obtain a vigorous culture with a sufficient number of inoculations
.
Generally, a rejuvenation process of 2 to 3 generations is required to allow the strain to gradually adapt to the culture environment
(1) The resuscitation of strains in the subculture preservation method is relatively simple, just transfer directly
.
(2) When the bacteria preserved in liquid paraffin is recovered, use an inoculating loop to pick a small amount of bacteria from under the paraffin oil, and lightly touch it on the tube wall a few times to make the oil drop as much as possible, and then transfer to a suitable fresh Medium
.
Because the bacteria are stained with paraffin oil, they grow slowly and are sticky, so they generally need to be transplanted again to get a good strain
(3) When the bacteria preserved by the sand tube method are recovered, open the sand tube under aseptic conditions, take part of the sand soil particles on a suitable slope medium, and transfer them again after the bacteria grow, or take the sand particles in a suitable In the liquid medium, the slope is transferred after propagation and cultivation
.
(4) When the strains preserved by the vacuum freeze-drying method are recovered, first wipe the upper part of the ampoule tube with 70% alcohol cotton, heat the upper part of the ampoule tube on the flame, use a sterile cotton swab dipped in sterile water, and wipe the top part.
Or drop a few drops of sterile water directly on the hot place to cause cracks in the tube wall.
Leave it for a while to allow air to slowly enter the tube from the crack, and then use a file or tweezers to knock off the cracked end, which can prevent sudden air blows.
Put the bacteria powder into the ampoule tube to fly
.
Then use a small amount of sterile water or culture solution to dissolve the bacterial mass to fully dissolve the dry bacterial powder, then use a sterile pipette to transfer to the fresh medium and place it at the optimum temperature for cultivation
(5) When liquid nitrogen ultra-low temperature freezing method preserves the bacterial species or -80 ℃ refrigerator freezing method preserves the bacterial species to recover, the ampoule tube or plastic cryotube should be immediately placed in a 38-40 ℃ water bath for rapid recovery and appropriately and quickly shaken until the inside It takes 50-100s until the icing is completely dissolved
.
Open the ampoule tube or plastic cryotube, and transfer the contents to a suitable medium for cultivation
(6) When the strains preserved by the magnetic bead method are recovered, directly take out 1 to 2 magnetic beads and put them in a petri dish to shake, or put the magnetic beads into a liquid medium for culture
Related links: Microbial strain preservation technology
