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    Home > Biochemistry News > Microbiology News > Studies have revealed a new mechanism for nitric oxide inhibition of Pseudomonas aeruginosa

    Studies have revealed a new mechanism for nitric oxide inhibition of Pseudomonas aeruginosa

    • Last Update: 2022-09-22
    • Source: Internet
    • Author: User
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    Pseudomonas aeruginosa (PA), also known as Pseudomonas aeruginosa, accounts for 10% to 15% of global nosocomial infections, the infection fatality rate of Pseudomonas aeruginosa is as high as about 38%, and the current treatment regimen is mainly focused on antibiotic treatment, and drug resistance is a challenge
    for treatment regimens.

    How to effectively inhibit Pseudomonas aeruginosa and reduce its resistance to antibiotics is a key problem
    in clinical treatment.

    In response to this scientific problem, Chen Chang, a researcher at the Institute of Biophysics of the Chinese Academy of Sciences, and Wan Yi, a researcher at the Shaanxi Institute of Microbiology, recently published a paper entitled Nitric oxide inhibits alginate biosynthesis in Pseudomonas aeruginosa and increases its sensitivity to The academic paper tobramycin by downregulating algU gene expression reveals a novel mechanism
    by inhibiting Pseudomonas aeruginosa by regulating the key factor Argeuilus Argulum by inhibiting PA alginate hypersynthesis.

      

    PA's argU is a sigma factor of extracellular function, which is responsible for the oversynthesis of alginate, which forms a thick mucus outside the cell, reducing the permeability of its outer membrane to produce resistance, contributing to the long-term survival
    of bacteria in chronic infections.

    The research team constructed a fluorescent reporter strain containing argU, monitoring argU content in real time under different conditions to characterize the growth
    of Pseudomonas aeruginosa.

    The study found that no treatment inhibited PA alginate synthesis: endogenous and exogenous NO treatments can significantly reduce the argU expression of PA, resulting in downregulation
    of a series of alginate synthesis-related genes (algD, alg8, algX, and algK).

    In both the model strain and the clinical PA isolate, NO inhibited the synthesis of PA alginate and was more pronounced
    on the mucous PA strain.

    Antibiotic susceptibility studies have found that in the presence of nitroprusside (SNP), a 10 μM NO donor, the number of free PA bodies increases and the sensitivity of adherent and free PA bodies to tobramycin increases
    to varying degrees.

    During infection in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) is susceptible to mucus-type strains, characterized by excessive production of alginates
    .

    The study suggests that NO may be used to prevent and treat PA infections in cystic fibrosis lungs, improving the sensitivity of strains to antibiotics, and has important implications
    for the clinical treatment of PA.

      

    The related research has been funded by the National Key Research and Development Program of Protein Machine and Life Process Regulation, the Strategic Pilot Science and Technology Project of the Chinese Academy of Sciences (Category B), the Major Research Program of the National Natural Science Foundation of China, the Youth Fund of the National Natural Science Foundation of China, and the Shaanxi Academy of Sciences
    .


    Pattern diagram of nitric oxide inhibition of Alginate hypersynthesis of Pseudomonas aeruginosa


    Source: Institute of Biophysics, Chinese Academy of Sciences



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