Study on the degradation of lignocellulose: The real role of Cel48S in fibrous small bodies.

Fiber small body is an efficient degradation of lignocellulose multi-enzyme complex secreted by bacteria, and its high-efficiency degradation mechanism and genetic modification of fiber-producing small body bacteria are one of the important directions in the study of lignocellulose degradation utilization. Cel48S,
thermoficoccus, is the main exosome glucanase of its fibrosis, the highest content of its fiber small body, and plays a key role in cellulose degradation.
but the intrinsic nature of Cel48S makes it very difficult to purify Cel48S, there are many contradictions in the literature on the nature of the enzyme identification report, and the enzyme's important role in the fibrous body is not consistent, which makes it difficult to reveal the high-efficiency degradation mechanism of fibrosis in depth.
, cui Ball and Feng Yingang, associate researcher of the metabolic matter group of Qingdao Institute of Bioenergy and Process Research, Chinese Academy of Sciences, based on the pre-developed thermal ficobacteria genetic modification tools, through the targeted transformation of the fiber small body, realized the efficient purification of its in situ Cel48S catalytic domain, thus successfully systematically analyzing the enzyme and structural properties of Cel48S, revealing the real role of 48S in the fiber small body.
results were published online in Biotechnology for Biofuels.
the first step of high-value utilization of lignocellulose biomass, and it is also a hot research topic at home and abroad.
in order to break the monopoly of foreign enzyme technology and break through the bottleneck of glycation technology, the atabmatic team is committed to developing a new integrated bioglytic technology route based on the small body of thermoficoccal fibrosis.
in this route framework, it is necessary to obtain an efficient whole bacteria catalyst through the mechanism analysis and orientation improvement of the fiber small body, a multi-enzyme complex.
for this purpose, the metabolite stomic team systematically developed non-modal microbial gene operation tools for Clostridium difficile, in the thermal fibrills to achieve rapid and accurate gene knockout, overexpression and precise editing, complete the hot Line Clostridium difficile fiber small body scaffold protein functional contribution, release fiber small body feedback inhibition of the targeted transformation and other series of work.
at the same time, the team established a mature protein structure analysis platform such as X-ray crystallography and biomolecular nuclear magnetic resonance, and completed the study of several fiber small body components and industrial enzyme catalysis mechanisms.
based on a sound genetic operating platform and a protein structure analysis platform, the researchers conducted a more systematic and in-depth study of Cel48S, a key component of the fibrous small body.
as the key enzyme component with the highest content in the cell fiber cell of clostridium, the enzymatic properties and structural characteristics of Cel48S have been widely concerned.
team revised the structure of the assembly module of Cel48S in the literature by MRI, and gained a new understanding of the assembly method of the fiber body.
for the catalytic module of Cel48S, due to the difficulty of heterogenic expression, the tight binding with the fibrous small body is difficult to separate from the in situ, the literature on its nature of the reported contradictions, the enzyme in the fiber small body of the real role has not been effectively clarified. in order to solve this problem
, the researchers used a pre-established precision genetic operation tool for clostridium difficile, which enabled the separation of Cel48S's catalytic domain from the fibrous gland and the direct purification of the step from the culture by inserting a sequence of coding histine labels and terminating the cryptosine behind the catalytic domain of Cel48S.
on this basis, through enzymatic determination and protein structure analysis, the high activity of natural Cel48S, the substrate preference of crystalline cellulose and the induced fit effect of the substrate coupling were determined, and the structural functional characteristics of Cel48S, the key component of the cell matter, were clarified.
at the same time, the study provides a method for isolating the fiber small body or other secretion proteins based on precise genetic operation to achieve the separation of the insituitis, and provides an example and basis for further study of the lignocellulose degradation mechanism of the fiber small body and the construction of a targeted improved strain of liglose glycation.
series of research has been supported by the National Science and Technology Support Program, the National Natural Science Foundation of China and the Construction Of the Alliance of Key Laboratory Of Sugar Industry Science and Technology in Shandong Province.
.