Study on the path and regulation mechanism of procedural cell death in plants.

Procedural cell death is a genetically regulated cell death method, which plays an important role in the growth and development of plants and animals and the resistance to biological and non-biological stress.
has been shown that chlorophyr and mitochondria play an important role in plant procedural cell death, but it is not clear whether there is signal communication between the two and how synergies can work together to regulate procedural cell death.
earlier, the Li Jiayang Research Group of the National Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences, cloned a amoeba cell death mutant mosaic death 1 (mod1).
MOD1 encodes an aphyl-ACP reductase, which is essential for fatty acid synthesis in chloroplasts.
the study of mod1 inhibition mutants found that there is active oxygen accumulation in mod1 mutants, while the decrease in activity of mitochondrial electron transfer chain I can inhibit the accumulation of reactive oxygen in mod1, thus inhibiting the occurrence of cell death.
this result suggests that there may be chlorophyll-to-mitochondria during cell death caused by THE MOD1 mutation.
by studying mod1 inhibitors that do not affect the activity of mitochondrial complex I, Li Jiayang's research team screened and cloned three new inhibitors: the NAD-dependent malic dehydrogenase (plNAD-MDH) for plasmid-positioned, the dioxic transport protein 1 (DiT1) where the chloroform is membrane-positioned, and the mitochondrial-positioned malic-dehydrogenase 1 (mMDH1).
all three genes are important members of the plant malic acid-oxalate shuttle pathway, and the loss of functionality of each gene inhibits the accumulation of reactive oxygen and cell death in mod1.
experimental results show that MOD1 function is reduced, resulting in a large amount of accumulation of its substrate NADH in the chloroplast, which in turn causes the magenta acid carrying reducing force to enter the mitochondria through the malic acid-oxalate acid shuttle, leading to the rise of NADH levels in the mitochondria, causing the production of reactive oxygen and cell death. Further research
showed that the pathway plays an important role in the production and oxidative stress of reactive oxygen caused by continuous light.
, malic acid treatment of HeLa cells can induce the production of reactive oxygen and cell death, and mitochondrial-positioned malic dehydrogenase plays an important role.
the results of this study demonstrate the presence of chloroplast-To-Mitochondria communication in the programmed cell death pathway of plants,the malic acid-oxalate acetic acid shuttle pathway plays a key role in it, and there is a conservative cytoplasmic-to-mitochondria cell death regulation mechanism in animals.
recently published the results online in Cell Research.
Li Jiayang Research Group Ph.D. ph.D. student Zhao Yannan, postdoctoral luo Lilan and Huang Xun Research Group Assistant Researcher Xu Jiesi as co-first authors of the paper, researcher Li Jiayang, researcher Huang Xun and associate researcher Yu Wei as co-authors.
collaborators include Dr. Yu Jinfang of the Institute of Genetic Development, Zuo Jianru, a researcher, and Wang Dong, a researcher.
research work has been supported by the National Natural Science Foundation of China project and the strategic leading science and technology project of the Chinese Academy of Sciences.
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