Successfully developed a green bio-manufacturing chassis cell high-efficiency editing tool

Successfully developed a green bio-manufacturing chassis cell high-efficiency editing tool

Successfully developed green bio-manufacturing chassis cell high-efficiency editing tool successfully developed green bio-manufacturing chassis cell high-efficiency editing tool

Zymomonas mobilis repairs damage to genomic DNA and movable elements caused by the CRISPR-Cas system
.

Photo courtesy of Central China Agricultural University

 Zymomonas mobilis repairs damage to genomic DNA and movable elements caused by the CRISPR-Cas system

Zymomonas mobilis( Zymomonas mobilis ) is a bacteria that can directly use the ED pathway to metabolize sugars to produce ethanol under anaerobic conditions.
It has the characteristics of high ethanol fermentation rate, good ethanol tolerance, and biosafety.
The application has great application value
.

Zymomonas mobilis

The research team found that the genomic DNA damage caused by the CRISPR-Cas system can be efficiently repaired by the micro-homology-mediated joining pathway (MMEJ), and part of the DNA is missing
.

The MMEJ system has a very low efficiency in repairing the damage of moving elements caused by CRISPR-Cas, which represents a recognition mode that distinguishes itself from foreign DNA after cutting

Journal of Genetics and Genomics

On this basis, the research group used the endogenous CRISPR-Cas system and the MMEJ approach to develop a tool suitable for rapid genome editing and essential gene identification of Z.
mobilis
.

The tool only needs to construct a self-targeting plasmid with mini-CRISPR to achieve the knockout of target genes and long fragments

Biotechnology for Biofuels

Pediococcus lactis is an important probiotic in the feed and medicine catalogs.
It is widely used in production practices and clinical treatments such as lactic acid and feed fermentation and immune regulation
.

The research team isolated a strain of Pediococcus lactis with good production performance and strong probiotic properties in the early stage, and found that its genome encodes the CRISPR-Cas9 system

Although the cas9 gene has a nonsense mutation, research by the research team has shown that it can still perform the nucleic acid cleavage function
.

Based on the endogenous CRISPR-Cas9 system, the research team has established efficient genetic manipulation tools to achieve efficient gene knockout, point mutation and integration

Applied and Environmental Microbiology

Related paper information:

https://doi.

https://doi.

https://doi.

https://doi.
org/10.
1128/AEM.
00948-21