Sugar-lipid MacCer promotes sudden-triggering by regulating the Wg signaling pathway.

Lipids, as cell membrane components and signal ingons, are essential to the development and function of the nervous system.
a variety of gene mutations involved in lipid metabolism lead to neurological disorders.
but there are many lipids and transsynthesis in anabolic pathways, which lipids participate in regulating neurodevelopment and its related regulatory mechanisms is a major scientific problem in the field of neurobiology. Zhang Yongqing, a researcher at the Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences,
, used traditional patterns of biological fruit flies as a material and found that several genes in the gsaccharide (GSL) synthesis pathway regulated the growth of nerve synapses.
further genetic analysis combined with liposome-specific antibody staining found that GSL glycolic-glucose ceramide (MacCer) promotes synaptic growth.
GSL is assembled into a fat raft with sterols on biofilms.
immunostaining shows MacCer and Wg in the synaptic co-positioning (top left); molecular simulationshows MacCer's binding to Wg (top right); and MacCer regulates the model of Wg signal and synaptic growth (bottom).
using pharmacology experiments combined with genetic analysis, it was found that MacCer's synaptic growth depended on the fat raft.
many proteins bind to lipo-rafts and participate in signal transduction, such as the growth factor Wnt1/Wingless (Wg) that binds to the resonant raft sesame and activates the signaling pathway to promote synaptic growth, and the activation of the Wg signaling pathway depends on its binding to the fat raft. further research
further studies have shown that MacCer is co-located with Wg in nerve synapses and is regulating Wg levels to affect The activity of the Wg signaling pathway.
to further study how MacCer interacts with Wg, they worked with Jacques Fantini, a professor at the University of Marseille in France, and found that a short peptide containing 15 amino acids in Wg could bind specifically to MacCer through molecular simulations and lipid biochemical tests.
further invivia experiments have shown that the combination of the two affects the co-location and regulation of the growth of nerve synapses by MacCer and Wg.
the first of its total, the study found that glycosacmac macCer promotes sudden-triggering by regulating the Wg signaling pathway.
the findings were published online October 25 in the international journal eLife.
Zhang Yongqing Laboratory postdoctoral huang Wei is the first author of the paper.
the research was supported by the Ministry of Science and Technology, the National Natural Science Foundation of China and the Chinese Academy of Sciences.
Source: Institute of Genetics and Developmental Biology.