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F.
Anion exchange column
Ito et al.
used Bond Elut PSASPE column to purify 10 SAs in animal liver and kidney
.
The sample was extracted with ethyl acetate , evaporated to dryness, and dissolved in 5mL ethyl acetate- n-hexane (50+50, v/v), purified by Bond Elut PSA SPE column, and purified with 5mL acetonitrile- 0.
05mol/L ammonium formate solution (20 +80, v/v) eluted and then detected by HPLC
Wu Yinliang waited until the establishment of a solid-phase extraction-reverse-phase HPLC method for the analysis of 7 SAs residues in chicken liver
.
The sample was extracted with ethyl acetate.
After the water was removed by anhydrous sodium sulfate , it was purified by an amino SPE column, rinsed with 2 mL of ethyl acetate, dried under vacuum, and then used 2 mL of ethyl acetate-methanol-water-acetic acid mixed solution (2+ 2+9+0.
G.
Cation exchange column
Lin Haidan and others established an HPLC method for the determination of 8 SAs residues in eel and its products
.
The sample was extracted with dichloromethane , dried with nitrogen, dissolved with 1.
0 mL of 1% acetic acid-methanol (65+35, v/v), added 3 mL of n-hexane LLP to degrease, and the lower layer was diluted to 3 mL with 5% acetic acid solution.
H.
Combined column
Tarbin et al.
reported the use of LC-MS and GC-MS to determine the residual analysis method of SAs in eggs
.
The sample was extracted with acetonitrile.
After acidification with acetic acid, it was purified by Bond-Elut SCX column (activated with 5% acetic acid acetonitrile).