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On August 28, the Journal of the National Academy of Sciences (PNAS) published an online paper entitled Mechanistic Insights Plant Plant HH3K9 methyltransferas and binding to their sitcom-in-the-gig, in collaboration with the Du Jiamu Research Group of the Shanghai Plant Adversity Biology Research Center of the Chinese Academy of Sciences and the Steven Jacobsen Research Group at the University of California, Los Angeles.
this paper has studied plant-specific SUVH family protease H3K9 methylaterase from many angles, from the perspectives of structural biology, biochemistry, genomics, etc., and found the regulatory mechanism of this enzyme, and successfully analyzed the molecular basis of plant CHG site methylation sequence preference.
DNA methylation is an important epigenetic marker, which is of great significance to maintain the stability of the genome.
plant DNA methylation is strictly regulated by histone modification H3K9me2. The methylation of H3K9 in
plants is mainly done by plant-specific SUVH family proteins, which bind to methylated DNA through its SRA domain and methylate histones through its SET domain.
however, the biochemical regulatory mechanism of this enzyme is rarely studied.
the study, the researchers first conducted structural biology studies on SUVH6, a member of the SUVH family, and determined the crystal structure of multiple states of the SUVH6 and the compounds of different combinations of DNA and cofactor SAM.
through structural analysis, it is found that the peptide substrate binding channel of SUVH6 is regulated by its own self-inhibition.
, the SUVH family's protein-binding methylation DNA is more dependent on its thumb loop, while UHRF1 in mammals is more dependent on NKR finger.
on this basis, the researchers determined the combination constant of three congenous redundant genes of SUVH4/5/6 and different sequences of methylation DNA, and found that THE main gene In-plant sane gene H3K9 methylation, SUVH4, which preferred CAG/CTG methylation, preferred CCG methylation, and SUVs H6, which successfully explained the reasons for CAG/CTG methylation significantly higher in the plant.
partner's genome-wide Chip-seq experiment also validates this explanation.
the study has greatly deepened the understanding of the regulation and in vivo sequence preference of THE SUVH family methylater. Li Xueqin, a graduate student at the
Plant Adversity Center, is the co-first author of the paper, and Du Jiamu and Steven Jacobsen are co-authors of the paper.
the research work is funded by the Ministry of Science and Technology's National Key Research and Development Program, the Fund Committee, the National Key Laboratory of Plant Molecular Genetics and the Chinese Academy of Sciences.
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