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Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles

 Last Update: 2020-12-26
 Source: Internet
 Author: User

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Gold nanoparticles functionalized with thiol-modified single-stranded oligonucleotides are highly useful reagents for a variety of applications, ranging from materials science to bioanalytics. In this chapter, the preparation of citrate stabilized 15-nm Au nanoparticles is described. The nanoparticles are conjugated with 3′-thiol-modified deoxyribonucleic acid oligomers and the resulting conjugates are characterized by determining their shape, size, and surface coverage. The hybridization capabilities are quantified in a microplate assay.

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