Synthesis of DNA Using a New Two-Step Cycle
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Last Update: 2020-11-26
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Source: Internet
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Author: User
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For the first time a new, two-step method is described for synthesizing deoxyribonucleic acid. The approach uses 5′-carbonate protected 2′-deoxynucleoside-3′-phosphoramidites as synthons and a peroxy anion buffer that removes the carbonate protecting group and oxidizes the internucleotide linkage. Following synthesis via this two-step cycle, oligomers are isolated by standard procedures.
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